Transgenic Populus hybrid expresses a wound-inducible potato proteinase inhibitor II - CAT gene fusion
Transgenic Populus hybrid expresses a wound-inducible potato proteinase inhibitor II - CAT gene fusion
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转基因杨杂种表达伤口诱导的马铃薯蛋白酶抑制剂 II - CAT 基因融合
DOI：
10.1139/x91-186
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发表时间：
1991
影响因子：
2.2
通讯作者：
R. Thornburg
中科院分区：
文献类型：
--
作者：
N. Klopfenstein;N. Shi;A. Kernan;H. Mcnabb;R. B. Hall;E. Hart;R. Thornburg
杂交杨树克隆已用先前构建的伤口诱导型马铃薯蛋白酶抑制剂 (pin2) – 氯霉素乙酰转移酶 (CAT) 嵌合基因转化，该基因与胭脂碱合酶 (nos) – 新霉素磷酸转移酶 II (NPT II) 选择标记基因相连。银白杨×大齿杨汉森克隆通过叶共培养的方式与含有双元载体的根癌农杆菌菌株A281进行转化。芽在含有硫酸卡那霉素的选择性培养基上再生并生根。随后，将植物种植在土壤中以供温室和田间生长。在叶子提取物中观察到 nos – NPT II 选择标记基因的 NPT II 活性，从而证实了转移的选择标记基因在杨树中的表达。 Southern 杂交证实了 pin2-CAT 结构的单个拷贝并入了转化的杂交杨树的基因组中。转基因杨叶的 Northern 分析表明，马铃薯 pin2-CA...
A hybrid poplar clone has been transformed with a previously constructed wound-inducible potato proteinase inhibitor (pin2) – chloramphenicol acetyltransferase (CAT) chimeric gene linked to a nopaline synthase (nos) – neomycin phosphotransferase II (NPT II) selectable marker gene. The Populusalba × Populusgrandidentata Hansen clone was transformed by means of leaf cocultivation with Agrobacteriumtumefaciens strain A281 containing a binary vector. Shoots were regenerated and rooted on selective medium containing kanamycin sulfate. Subsequently, plants were established in soil for greenhouse and field growth. NPT II activity from the nos – NPT II selectable marker gene was observed in leaf extracts, thereby confirming expression of the transferred selectable marker gene in poplar. Southern hybridization confirmed the incorporation of a single copy of the pin2–CAT construction into the genome of the transformed hybrid poplars. Northern analysis of transgenic poplar leaves demonstrated that the potato pin2–CA...