Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo
Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo
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DOI：
10.1117/1.3589299
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发表时间：
2011-06-01
影响因子：
3.5
通讯作者：
Farkas, Daniel L.
中科院分区：
文献类型：
--
作者：
Hwang, Jae Youn;Gross, Zeev;Farkas, Daniel L.
我们报告了一种新的在体光谱成像方法的癌症检测和化疗评估。我们描述和表征比率光谱成像和分析方法，并通过定量监测靶向镓可咯（HerGa）在HER2阳性（HER2+）乳腺肿瘤中的特异性积累来评估其用于肿瘤检测和描绘的性能。通过a.这种新的比率成像和分析方法; B.建立的（反射率和荧光）光谱成像; c.更常用的荧光强度成像。我们还测试了HerGa成像的可行性，在体内使用的比率光谱成像方法的肿瘤检测和划定。我们的研究结果表明，新方法不仅提供了比典型的光谱成像更好的定量信息，而且比标准荧光强度成像更好的特异性，从而允许增强肿瘤的体内轮廓和动态，定量监测靶向化疗药物的积累。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI 10.1117/1.3589299]
We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2+) breast tumors. HerGa temporal accumulation in nude mice bearing HER2+ breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3589299]