Rapid identification of nontypeable and serotype b Haemophilus influenzae from nasopharyngeal secretions by the multiplex PCR
Rapid identification of nontypeable and serotype b Haemophilus influenzae from nasopharyngeal secretions by the multiplex PCR
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DOI：
10.1016/j.ijporl.2006.10.009
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发表时间：
2007-02-01
影响因子：
1.5
通讯作者：
Yamanaka, Noboru
中科院分区：
文献类型：
--
作者：
Billal, Dewan S.;Hotomi, Muneki;Yamanaka, Noboru
目的：研究流感嗜血杆菌（H.流感）是造成侵入性和非侵入性传染病的重要病原体。虽然被封装的B型菌株被认为是严重侵袭性疾病的主要原因，但不可分型菌株是非侵袭性感染性疾病的主要原因。方法：采用多重聚合酶链反应（PCR）技术，对鼻咽分泌物中的B H型和非分型型的病原体进行快速鉴定。结果：共检测25例鼻咽分泌物。结论：多重PCR血清学分型方法比玻片凝集试验（SAT）结果更可靠，是一种有价值的快速鉴定方法。确定荚膜血清型。(C)2006爱思唯尔爱尔兰有限公司保留所有权利。
Objective: Heamophilus influenzae (H. influenzae) is an important pathogen responsible for both invasive and non-invasive infectious diseases. While encapsulated type b strain recognized as a major cause of severe invasive diseases, nontypeable strains are the major causes of non-invasive infectious diseases. Detection of this pathogen from nasopharyngeal secretions (NIPS) is important.Methods: We developed a multiplex polymerase chain reaction (PCR) for rapid identification of nontypeable and serotype b H. iniluenzae from nasopharyngeal secretions.Results: A total 25 nasopharyngeal secretions were evaluated in this study. The multiplex PCR provided rapid and unequivocal results for determining either nontypeable or encapsulated typeable especially type b strains including a determination of beta-lactamase productions.Conclusion: The multiplex PCR based serotyping provided more reliable results than slide agglutination test (SAT) and is a valuable and expeditious method for identification of H. influenzae with determining capsular serotypes. (C) 2006 Elsevier Ireland Ltd. All rights reserved.