P-SELECTIN INDUCES THE EXPRESSION OF TISSUE FACTOR ON MONOCYTES
P-SELECTIN INDUCES THE EXPRESSION OF TISSUE FACTOR ON MONOCYTES
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DOI：
10.1073/pnas.91.19.8767
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发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
FURIE, B
中科院分区：
文献类型：
--
作者：
CELI, A;PELLEGRINI, G;FURIE, B
活化血小板和刺激内皮细胞上的P选择素介导细胞与单核细胞和中性粒细胞的粘附。由于活化的血小板诱导单核白细胞上的组织因子，我们研究了P-选择素对单核细胞中组织因子活性表达的影响。纯化的P-选择素以剂量依赖性方式刺激单核白细胞上的组织因子表达。表达P-选择素的中国仓鼠卵巢（CHO）细胞刺激纯化的单核细胞中的组织因子促凝活性，而未转染的CHO细胞和表达E-选择素的CHO细胞则没有。抗P-选择素抗体抑制纯化的P-选择素和表达P-选择素的CHO细胞对单核细胞的作用。表达P-选择素的CHO细胞与单核细胞孵育导致单核细胞中组织因子mRNA的产生和单核细胞表面组织因子抗原的表达。这些结果表明，P-选择素上调单核细胞上的组织因子的表达，以及介导血小板和内皮细胞与单核细胞和中性粒细胞的结合。P选择素与血管损伤区域单核细胞的结合可能是引发血栓形成的机制的一个组成部分。
P selectin on activated platelets and stimu lated endothelial cells mediates cell adhesion with monocytes and neutrophils. Since activated platelets induce tissue factor on mononuclear leukocytes, we examined the effect of P-selectin on the expression of tissue factor activity in monocytes. Purified P-selectin stimulated tissue factor expression on mononuclear leukocytes in a dose dependent manner. Chinese hamster ovary (CHO) cells expressing P-selectin stimulated tissue factor procoagulant activity in purified monocytes, whereas untransfected CHO cells and CHO cells expressing E-selectin did not. Anti-P-selectin antibodies inhibited the effects of purified P-selectin and CHO cells expressing P-selectin on monocytes. Incubation of CHO cells expressing P-selectin with monocytes leads to the development of tissue factor mRNA in monocytes and to the expression of tissue factor antigen on the monocyte surface. These results indicate that P-selectin upregulates the expression of tissue factor on monocytes as well as mediates the binding of platelets and endothelial cells with monocytes and neutrophils. The binding of P selectin to monocytes in the area of vascular injury may be a component of a mechanism that initiates thrombosis.