Autoantibodies against inner ear proteins in patients with delayed endolymphatic hydrops and unilateral juvenile deafness
Autoantibodies against inner ear proteins in patients with delayed endolymphatic hydrops and unilateral juvenile deafness
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DOI：
10.1080/00016480500266008
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发表时间：
2006-02-01
影响因子：
1.4
通讯作者：
Kitano, H
中科院分区：
文献类型：
--
作者：
Suzuki, M;Hanamitsu, M;Kitano, H
结论.对侧型迟发性内淋巴积水（DEH）的患者可能会对另一个内耳进行自身免疫攻击。由于单侧青少年性耳聋患者尽管观察时间较长，但无进展，因此抗68-kDa蛋白的自身抗体可能与DEH的发病机制无关。Objective.对侧型的DEH被认为具有自身免疫性病因，有时由单侧青少年耳聋发展而来。本研究的目的是确定自身抗体是否在DEH中具有重要的病理意义。材料和方法。应用Western blot方法检测了9例DEH患者、18例极重度单侧青少年耳聋患者和15例正常人血清中的内耳蛋白。结果8例对侧型DEH中6例（75%）在Western blotting上至少有1条反应带。反应最频繁的蛋白质分子量为28 kDa，这与我们以前的结果一致。在18名单侧青少年耳聋患者中，5名（28%）显示反应性条带，仅在68 kDa处。
Conclusions. Patients with the contralateral type of delayed endolymphatic hydrops ( DEH) may undergo an autoimmune attack against the other inner ear. As patients with unilateral juvenile deafness show no progression, despite lengthy observation, the autoantibody against the 68-kDa protein may be unrelated to the pathogenesis of DEH. Objective. The contralateral type of DEH is believed to have an autoimmune etiology, and sometimes develops from unilateral juvenile deafness. The purpose of this study was to determine whether autoantibodies are pathogenetically important in DEH. Material and methods. Sera from 9 patients with DEH, 18 patients with profound unilateral juvenile hearing loss and 15 control volunteer without inner ear diseases were investigated by means of Western blot assay against rat inner ear proteins. Results. Among 8 patients with the contralateral type of DEH, 6 (75%) showed at least 1 reactive band on Western blotting. The protein that reacted most frequently had a molecular weight of 28 kDa, which was consistent with our previous results. Among 18 patients with unilateral juvenile deafness, 5 ( 28%) showed reactive bands, exclusively at 68 kDa.