Supplementation with asiatic acid during in vitro maturation improves porcine oocyte developmental competence by regulating oxidative stress
Supplementation with asiatic acid during in vitro maturation improves porcine oocyte developmental competence by regulating oxidative stress
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体外成熟过程中补充积雪草酸可通过调节氧化应激改善猪卵母细胞的发育能力
DOI：
10.1016/j.theriogenology.2021.06.013
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发表时间：
2021
期刊：
影响因子：
2.8
通讯作者：
Liang Shuang
中科院分区：
文献类型：
--
作者：
Qi Jia-Jia;Li Xiao-Xia;Zhang Yan;Diao Yun-Fei;Hu Wei-Yi;Wang Da-Li;Jiang Hao;Zhang Jia-Bao;Sun Bo-Xing;Liang Shuang
积雪草酸是积雪草中发现的一种天然三萜类化合物，具有有效的自由基清除剂作用。我们先前的研究表明，积雪草酸可延缓猪卵母细胞体外老化，提高体外着床前胚胎发育能力；然而，积雪草酸在猪卵母细胞成熟过程中对氧化应激的保护作用尚不清楚。在此，我们研究了积雪草酸对猪卵母细胞体外成熟(IVM)和孤雌激活(PA)和体外受精(IVF)后胚胎发育能力的影响。本研究结果表明，添加10 mM积雪酸不影响猪卵母细胞卵丘细胞的扩张和极体的排出，而显著提高猪PA和IVF胚胎的后续囊胚率和质量。过氧化氢(H_2O_2)是一种引起猪卵母细胞氧化应激的活性氧物种。正如预期的那样，补充亚麻酸不仅降低了细胞内ROS水平，而且还减弱了过氧化氢诱导的细胞内ROS的产生。进一步的分析表明，补充亚麻酸可以增加细胞内谷胱甘肽的生成、线粒体膜电位和体外成熟末期的三磷酸腺苷的生成。综上所述，我们的研究结果表明，在猪卵母细胞体外成熟过程中，补充亚麻酸通过调节氧化应激对卵母细胞产生有益的影响，并可能作为一种潜在的抗氧化剂应用于体外成熟的猪卵母细胞。
Asiatic acid is a natural triterpene found in Centella asiatica that acts as an effective free radical scavenger. Our previous research showed that asiatic acid delayed porcine oocyte ageing in vitro and improved preimplantation embryo development competence in vitro; however, the protective effects of asiatic acid against oxidative stress in porcine oocyte maturation are still unclear. Here, we investigated the effects of asiatic acid on porcine oocyte in vitro maturation (IVM) and subsequent embryonic development competence after parthenogenetic activation (PA) and in vitro fertilization (IVF). The results of the present research showed that 10 mM asiatic acid supplementation did not affect the expansion of cumulus cells or polar body extrusion of porcine oocytes, while asiatic acid application significantly increased the subsequent blastocyst formation rate and quality of porcine PA and IVF embryos. Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that induces oxidative stress in porcine oocytes. As expected, asiatic acid supplementation not only decreased intracellular ROS levels but also attenuated H2O2-induced intracellular ROS generation. Further analysis revealed that asiatic acid supplementation enhanced intracellular glutathione production, mitochondrial membrane potential, and ATP generation at the end of IVM. In summary, our results reveal that asiatic acid supplementation exerts beneficial effects on porcine oocytes by regulating oxidative stress during the IVM process and could act as a potential antioxidant in porcine oocytes matured in vitro production systems.