Activation of U937 cells by contact sensitizers: CD86 expression is independent of apoptosis.
Activation of U937 cells by contact sensitizers: CD86 expression is independent of apoptosis.
复制标题
DOI：
10.1080/15476910600978038
复制
发表时间：
2006-12-01
影响因子：
3.3
通讯作者：
Rousset, Francoise
中科院分区：
文献类型：
--
作者：
Ade, Nadege;Martinozzi-Teissier, Silvia;Rousset, Francoise
在树突状细胞和骨髓细胞系中由接触致敏剂诱导的不同表型变化中，CD 86似乎是一致的标志物，因为一直被描述为系统性上调。为了评价该标记物的稳健性，在U937骨髓单核细胞系中研究了细胞毒性对CD 86表达的干扰。在这项研究中，在用DNCB、NiSO（4）和pPD处理后48小时（CD 86表达的读取时间）观察到的细胞毒性显示是由在较早时间点发生的细胞凋亡引起的。这种变应原诱导的细胞凋亡至少部分依赖于半胱天冬酶，如响应DNCB和NiSO的半胱天冬酶-3激活以及Z-VAD-fatal抑制DNCB诱导的细胞凋亡所证明的。抑制细胞凋亡并没有改变DNCB处理的细胞中CD 86表达的刺激指数，表明细胞凋亡并不干扰CD 86表达的上调。此外，在DNCB处理的细胞中发现了相似的CD 86表达水平，无论是从包括凋亡细胞的整个非坏死细胞群体计算还是仅从活的非凋亡细胞群体计算。总之，这些结果证明参与死亡过程的细胞的存在不是响应于接触致敏剂的CD 86表达的混淆因素。他们还指出，细胞凋亡是细胞对接触致敏剂反应的另一个可能的关键标志。
Among the different phenotypic changes induced by contact sensitizers in dendritic cells and myeloid cell lines, CD86 appears to be a consensus marker, since constantly described as systematically up-regulated. To evaluate the robustness of this marker, interference of cytotoxicity on CD86 expression was investigated in U937 myelomonocytic cell line. In this study, cytotoxicity observed at 48 hr (reading-time for CD86 expression) after treatment with DNCB, NiSO(4) and pPD was shown to result from apoptosis taking place at earlier time points. This allergen-induced apoptosis was at least partly caspase-dependent as demonstrated by caspase-3 activation in response to DNCB and NiSO(4) and inhibition of DNCB-induced apoptosis by Z-VAD-fmk. Inhibition of apoptosis did not modify the stimulation index of CD86 expression in DNCB-treated cells, indicating that apoptosis did not interfere with up-regulation of CD86 expression. In addition, similar CD86 expression level was found in DNCB-treated cells whether calculated from the whole non-necrotic cell population including apoptotic cells or from viable non-apoptotic cell population only. Altogether, these results brought evidence that the presence of cells engaged in death process are not a confusing factor for CD86 expression in response to contact sensitizers. They also pointed out apoptosis as another possible key marker of cellular response to contact sensitizers.