Bacteria attached to suspended particles in Northern White Shrimp (Penaeus vannamei L.) ponds
Bacteria attached to suspended particles in Northern White Shrimp (Penaeus vannamei L.) ponds
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DOI：
10.1016/j.aquaculture.2005.04.039
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发表时间：
2005-09
期刊：
影响因子：
4.5
通讯作者：
Wang Yanbo;Xu Zirong;Zhou Xu-xia;X. Meisheng
中科院分区：
文献类型：
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作者：
Wang Yanbo;Xu Zirong;Zhou Xu-xia;X. Meisheng
对浙江海盐养殖北白对虾（Penaeus vannamei L.）池塘中附着在悬浮颗粒上的表层细菌进行了研究。采用4个不同表面积和年龄的池塘。采样日的水温为24 ~ 32℃。2004年5月20日至8月20日试验期间，各对虾池盐度为10 ~ 20‰，pH值为7.8 ~ 8.4。本研究测定了悬浮颗粒（SP）、附着细菌颗粒（BP）、表面细菌（即仅附着在悬浮颗粒上的细菌）和总平板数（TPC），并测定了颗粒有机碳（POC）浓度。此外，还分析了颗粒、表层细菌与POC的相关性。结果表明，4个虾池中SP的平均数量为5.41×104ml−1，BP为3.00×104ml−1，而附着细菌的颗粒占悬浮颗粒总数的55.41%。4个池塘的EB和TPC分别为6.33×104per ml和3.59×106per ml， EB占TPC的比例仅为1.76%。1年养殖池各阶段BP、EB和TPC均极显著降低（P<0.01）。4个虾池间POC差异不显著（P < 0.01）。1个、2个、3个和4个细菌附着的颗粒数量分别占细菌附着颗粒总数的46%、22%、14%和9%。然而，附着4个以上细菌的颗粒的百分比只有9%。表面细菌数量与附着细菌颗粒呈极显著相关（r=0.9920, n=36； P<0.01）。表层细菌数量与悬浮颗粒数（SP） （r=0.9558, n=36； P<0.01）和细菌总数（TPC） （r=0.8657, n=36； P<0.01）相关，与POC （r=0.1340, n=36； P< 0.05）不相关。
Epibacteria, i.e. bacteria attached to suspended particles, were studied in ponds, used for the cultivation of Northern White Shrimp (Penaeus vannamei L.), at Hai-yan Farm (Zhejiang Province, China). Four ponds with different surface area and age were used. The water temperature ranged from 24 to 32 °C on the sampling days. Salinity and pH values of all shrimp ponds ranged 10–20‰ and 7.8–8.4, respectively, during the experimental period, from 20 May to 20 August 2004. In this study, the numbers of suspended particles (SP), particles with bacteria attached (BP), epibacteria, i.e. bacteria attached to suspended particles only (EB), and total plate count (TPC) were determined and the concentration of particulate organic carbon (POC) measured. In addition, the correlation between particles, epibacteria and POC was analyzed. Results showed that the average number of SP in four shrimp ponds was 5.41×104ml−1, with a BP of 3.00×104ml−1, while the percentage of particles with bacteria attached was 55.41% of the total number of suspended particles. The EB and TPC of the four ponds were 6.33×104per ml and 3.59×106per ml, respectively, while the percentage of EB to TPC was only 1.76%. There were highly significantly lower counts (P<0.01) of BP, EB and TPC for the first year culture ponds during all phases. However, there was no significant difference (P>0.01) for POC between the four shrimp ponds. The number of particles to which one, two, three or four bacteria were attached was 46%, 22%, 14% and 9%, respectively, of the total number of particles with bacteria attached. However, the percentage of particles with more than four bacteria attach was only 9%. There was a significant correlation between the number of epibacteria and particles with bacteria attached (r=0.9920, n=36; P<0.01). The number of epibacteria was also correlated with the number of suspended particles (SP) (r=0.9558, n=36; P<0.01) and the total number of bacteria (TPC) (r=0.8657, n=36; P<0.01), but was not correlated with POC (r=0.1340, n=36; P>0.05).