Mass spectral analysis and imaging of tissue by ToF-SIMS -: The role of buckminsterfullerene, C60+, primary ions
Mass spectral analysis and imaging of tissue by ToF-SIMS -: The role of buckminsterfullerene, C60+, primary ions
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DOI：
10.1016/j.ijms.2006.09.015
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发表时间：
2007-02-01
影响因子：
1.8
通讯作者：
Vickerman, John C.
中科院分区：
文献类型：
--
作者：
Jones, Emrys A.;Lockyer, Nicholas P.;Vickerman, John C.
解吸/电离质谱技术的最新发展使其在生物分析中的应用成为现实和成功的主张。一次离子源技术的发展，主要是通过多原子离子束的出现，意味着二次离子质谱技术（SIMS）现在可以获得所需的信息深度，从而使生物成像成为一种可行的选择。在这里，主要离子C-60（+）的作用是评估关于脂质和药物在组织切片的分子成像。高二次离子产率和低表面损伤积累在模型和真实的生物样品上都得到了证明，表明与成像中使用的其他簇离子束相比，该一次离子为分析人员提供了高的二次离子效率。新开发的40 keV C-60（+）离子源允许光束聚焦，从而在组织样品上显示高分辨率成像，并且更高的产量允许来自药物raclopride的分子信号在体内给药后在组织切片内成像。该药物的定位暗示了有关影响分子电离概率的化学环境的问题；用模型系统证明了这种效应的重要性，并论证和讨论了使用激光后电离作为减少生物样品复杂性后果的方法的可能性。(C) 2006 Elsevier B.V.版权所有
Recent developments in desorption/ionisation mass spectrometry techniques have made their application to biological analysis a realistic and successful proposition. Developments in primary ion source technology, mainly through the advent of polyatomic ion beams, have meant that the technique of secondary ion mass spectrometry (SIMS) can now access the depths of information required to allow biological imaging to be a viable option.Here the role of the primary ion C-60(+) is assessed with regard to molecular imaging of lipids and pharmaceuticals within tissue sections. High secondary ion yields and low surface damage accumulation are demonstrated on both model and real biological samples, indicating the high secondary ion efficiency afforded to the analyst by this primary ion when compared to other cluster ion beams used in imaging. The newly developed 40 keV C-60(+) ion source allows the beam to be focused such that high resolution imaging is demonstrated on a tissue sample, and the greater yields allow the molecular signal from the drug raclopride to be imaged within tissue section following in vivo dosing.The localisation shown for this drug alludes to issues regarding the chemical environment affecting the ionisation probability of the molecule; the importance of this effect is demonstrated with model systems and the possibility of using laser post-ionisation as a method for reducing this consequence of bio-sample complexity is demonstrated and discussed. (C) 2006 Elsevier B.V. All rights reserved.