Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm
Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm
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DOI：
10.1016/s0093-691x(99)00228-9
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发表时间：
1999-12-01
期刊：
影响因子：
2.8
通讯作者：
Fugger, EF
中科院分区：
文献类型：
--
作者：
Fugger, EF
许多分离人类X和Y精子的方法已经报道，DNA分析后分离未经证实，出生结果不一致。成功的流式细胞术分离精子导致年轻出生的性别比的改变已在几种动物中得到证实。通过荧光原位杂交（FISH）进行DNA分析后，已证实了流式细胞术分离人类携带X和Y的精子（MicroSort（R））（a）。子宫内授精（IUI）、体外受精（IVF）和卵胞浆内单精子注射（ICSI）后使用MicroSort可导致妊娠和分娩。(C)1999年，Elsevier Science Inc.
Numerous methods to separate human X- and Y-bearing sperm have been reported with unconfirmed separation after DNA analysis and inconsistent birth results. Successful flow cytometric separation of sperm resulting in alteration of the sex ratio of young born has been demonstrated in several animal species. Flow cytometric separation of human X- and Y-bearing sperm (MicroSort(R))(a) has been confirmed after DNA analysis by fluorescence in situ hybridization (FISH). Pregnancies and births have resulted from the use of MicroSort after intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). (C) 1999 by Elsevier Science Inc.