Expression and function of members of the cytokine receptor superfamily on breast cancer cells
Expression and function of members of the cytokine receptor superfamily on breast cancer cells
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DOI：
10.1038/sj.onc.1200882
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发表时间：
1997-02-13
期刊：
影响因子：
8
通讯作者：
Begley, CG
中科院分区：
文献类型：
--
作者：
Douglas, AM;Goss, GA;Begley, CG
细胞因子白血病抑制因子（LIF）、白细胞介素-6（IL-6）、制瘤素M（OSM）、睫状神经营养因子（CNTF）和白细胞介素-11（IL-11）的受体是结构保守的促红细胞生成素受体超家族的成员。此外，它们都共享跨膜信号蛋白gp 130，在本文中，检测了该受体家族在乳腺癌细胞中的表达和功能，RT-PCR分析表明，gp 130在12/12乳腺细胞系中表达，IL-6，LIF，IL-11和CNTF在大多数这些细胞系中表达，这与其他造血素受体相反。通过RT-PCR检测50个恶性乳腺组织的临床样品显示出类似的gp 130相关受体表达模式，用OSM处理乳腺癌细胞系导致细胞形态的变化。细胞增殖受到抑制后，暴露于OSM（3/4细胞系），IL-11（2/4细胞系），并通过IL-6和LIF（1/4细胞系），还记录了LIF和OSM的细胞表面结合，这些受体在12/12的细胞系中表达，并且大于95%的细胞系中表达这些受体。临床样本的研究表明，这些分子可能在调节乳腺细胞的生长方面很重要。
Receptors for the cytokines leukemia inhibitory factor (LIF), interleukin-6 (IL-6), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and interleukin-11 (IL-11) are members of the structurally conserved hemopoietin receptor superfamily, In addition, they all share the transmembrane signalling protein gp130, In this paper the expression and function of this family of receptors in breast cancer cells was examined, RT-PCR analyses demonstrated that gp130 was expressed in 12/12 breast cell lines and the specific receptor alpha-chains for IL-6, LIF, IL-11 and CNTF were expressed in the majority of these cell lines, This was in contrast to other hemopoitin receptors, Examination of 50 clinical samples of malignant breast tissue by RT-PCR showed a similar pattern of expression of gp130 associated receptors, Treatment of breast cancer cell lines with OSM resulted in changes in cellular morphology. Cellular proliferation was inhibited following exposure to OSM (3/4 cell lines), IL-11 (2/4 cell lines), and by IL-6 and LIF (1/4 cell lines), Cell surface binding of LIF and OSM was also documented, The expression of these receptors in 12/12 cell lines and greater than 95% of clinical samples suggests that these molecules may be important in regulating the growth of breast cells.