Runx3 is essential for the target-specific axon pathfinding of trkC-expressing dorsal root ganglion neurons
Runx3 is essential for the target-specific axon pathfinding of trkC-expressing dorsal root ganglion neurons
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DOI：
10.1016/s1079-9796(03)00032-9
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发表时间：
2003-03-01
影响因子：
2.3
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Inoue, K;Ozaki, S;Ito, Y
背根神经节（DRG）神经元根据其感觉形态将轴突投射到脊髓灰质的特定目标层(Neuron 30 (2001) 707；Cell 101 (2000), 485；神经元31 (2001),59；中华神经科杂志，2004 (3),391；《感觉神经元》，牛津大学出版社。纽约，1992年，第131页)。Runx/AML基因的表达。Dev. 109(2001), 413)对DRG神经元亚型的研究表明它们参与了层特异性传入分化和成熟。我们发现Runx3-/-小鼠表现出严重的肢体共济失调和姿势异常，大多数小鼠在出生后不久死亡。结果表明，本体感觉传入轴突不能到达腹角，脊髓的背索也较小。尽管这种表型与缺乏神经营养因子-3 （NT-3）的敲除小鼠的表型非常相似(Cell 77 (1994), 503；Nature 369(1994), 658)及其受体trkC （Nature 368(1994), 249）通过选择性神经元细胞死亡显示本体感觉传入丢失，Runx3-/-小鼠在整个发育过程中保持正常数量的trkC /trk-C阳性DRG神经元。我们的研究结果表明，Runx3控制了脊髓中表达trkc的DRG神经元的目标特异性轴突寻路。(C) 2003 Elsevier Science（美国）版权所有。
Dorsal root ganglion (DRG) neurons project their axons to specific target layers in the gray matter of the spinal cord, according to their sensory modality (Neuron 30 (2001) 707; Cell 101 (2000), 485; Neuron 31 (2001), 59; J. Comp. Neurol. 380 (1997), 215; Sensory Neurons, Oxford Univ. Press. New York, 1992, p. 131). Expression of runt-related Runx/AML genes (Mech. Dev. 109 (2001), 413) on subtypes of DRG neurons suggests their involvement in lamina-specific afferent differentiation and maturation. Here we show that Runx3-/- mice display severe limb ataxia and abnormal posture and that most of them die shortly after birth. They show that proprioceptive afferent axons fail to reach the ventral horn and have a smaller dorsal funiculus in their spinal cords. Despite the strong resemblance of this phenotype to that of knockout mice deficient in neurotrophin-3 (NT-3) (Cell 77 (1994), 503; Nature 369 (1994), 658) and its receptor, trkC, (Nature 368 (1994), 249), which show proprioceptive afferent loss through selective neuronal cell death, Runx3-/- mice maintain normal number of TrkC/trk-C positive DRG neurons throughout development. Our results suggest that Runx3 controls the target-specific axon pathfinding of trkC-expressing DRG neurons in the spinal cord. (C) 2003 Elsevier Science (USA). All rights reserved.