CDC20 and its downstream genes: potential prognosis factors of osteosarcoma
CDC20 and its downstream genes: potential prognosis factors of osteosarcoma
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CDC20及其下游基因：骨肉瘤的潜在预后因素
DOI：
10.1007/s10147-019-01500-3
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发表时间：
2019-11-01
影响因子：
3.3
通讯作者：
Chen, Jia-xu
中科院分区：
文献类型：
--
作者：
Wu, Man-si;Ma, Qing-yu;Chen, Jia-xu
背景：利用基因芯片数据GSE42352筛选骨肉瘤预后相关基因。方法采用基因本体学(GO)生物学过程分析和京都基因与基因组百科全书(KEGG)分析方法对不同基因的功能进行验证。用实时荧光定量聚合酶链式反应(Real Time-PCR)验证芯片结果。结果通过对基因芯片GSE42352的分析，发现有丝分裂中期至后期相关基因CDC20、Securin、Cyclin A2和Cyclin B2在骨肉瘤细胞系中高表达。Kaplan-Meier生存分析表明，这些基因的过度表达可以预测骨肉瘤患者的不良预后。结论CDC20及其下游底物Securin、Cyclin A2和Cyclin B2是预测骨肉瘤预后的良好因子。这四个基因的任意两个组合作为骨肉瘤预后因子效果更好。
Background We investigated the microarray data GSE42352 to identify genes that can be used as prognosis factors in osteosarcoma.Methods Gene Ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of Cytoscape ClueGo were used in verifying the function of different genes. Realtime-PCR were used to confirm the microarray results. 83 patient samples were collected and underwent Kaplan-Meier survival analysis and multivariate analysis to predict the prospect of genes using as prognosis factors.Results After analyzing the microarray data GSE42352, mitosis metaphase to anaphase-related genes CDC20, securin, cyclin A2 and cyclin B2 were found to be overexpressed in osteosarcoma cell lines. Kaplan-Meier survival analysis showed that overexpression of these genes can predict poor prognosis outcomes in osteosarcoma patients. Furthermore, any combination of the four genes seems to be more effective in predicting osteosarcoma outcomes than any of these genes alone.Conclusions CDC20 and its downstream substracts securin, cyclin A2 and cyclin B2 are good factors that can predict prognosis outcomes in osteosarcoma. Any two combination of these four genes are more effective to be used as osteosarcoma prognosis factors.