Proliferation of dental follicle-derived cell populations in heat-stress conditions.
Proliferation of dental follicle-derived cell populations in heat-stress conditions.
复制标题
DOI：
10.1111/j.1365-2184.2011.00778.x
复制
发表时间：
2011-10
影响因子：
8.5
通讯作者：
Wise GE
中科院分区：
文献类型：
--
作者：
Yao S;Gutierrez DL;He H;Dai Y;Liu D;Wise GE
成体干细胞（ASC）的分离和纯化是一个巨大的挑战。我们的目标是确定 ASC 是否比非干细胞更耐热，并探索 ASC 是否可以通过热应激治疗来富集。将大鼠牙囊细胞在不同的培养基中培养，以获得由干细胞和非干细胞组成的异质细胞群（H-DFC），或仅包含非干细胞的同质细胞群（DFC）。进行实时 RT-PCR 来比较两个群体之间热休克蛋白 (HSP) 的表达。为了研究耐热性，将 H-DFC 和 DFC 在热应激条件下孵育，并通过阿拉玛蓝还原测定评估细胞增殖。此外，还评估了热应激处理产生的细胞的分化能力和干细胞标记物的表达。 H-DFC 表达的 HSP110、HSP70 和 HSP27 水平高于 DFC。与在 37°C 下生长的对照相比，H-DFC 在 40°C 下增加了增殖，并且在低于 40.5°C 的温度下没有发生增殖的显着减少。相比之下，DFC 在所有热应激处理下均显示增殖显着减少。此外，热应激的 H-DFC 增加了分化能力并增加了干细胞标志物的表达。干细胞似乎比非干细胞更能耐受热应激。在热应激条件下孵育异质细胞群导致干细胞数量增加。
Isolation and purification of adult stem cells (ASC) are a great challenge. Our objectives were to determine if ASC are more heat-tolerant than non-stem cells, and to explore if ASC can be enriched by heat-stress treatments. Rat dental follicle cells were cultured in different media to obtain either a heterogeneous cell population (H-DFC) consisting of stem cells and non-stem cells, or a homogenous cell population (DFC) containing only non-stem cells. Real-time RT-PCR was conducted to compare the expression of heat shock proteins (HSPs) between the two populations. To study the heat tolerance, H-DFC and DFC were incubated under heat stress conditions and cell proliferation was evaluated by an Alamar blue reduction assay. Furthermore, the cells resulting from heat stress treatments were evaluated for their differentiation capability and expression of stem cell markers. H-DFC expressed higher levels of HSP110, HSP70s and HSP27s than did the DFC. H-DFC increased proliferation at 40°C as compared to the control grown at37°C, and no significant reduction of proliferation occurred at temperatures below 40.5°C. In contrast, DFC showed significant reductions in proliferation under all heat stress treatments. Moreover, heat stressed H-DFC increased differentiation capability and increased expression of stem cell markers. Stem cells appear to be more tolerant to heat-stress than non-stem cells. Incubation of heterogeneous cell population in heat-stress conditions resulted in increased stem cell numbers.
登录
查看更多内容
影响因子：
3.7
作者：
Nishide K;Nakatani Y;Kiyonari H;Kondo T
通讯作者：
Kondo T
DOI：
10.1126/science.1180794
发表时间：
2010-01-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li L;Clevers H
通讯作者：
Clevers H
影响因子：
4.8
作者：
Krishnamurthy, P;Ross, DD;Schuetz, JD
通讯作者：
Schuetz, JD
影响因子：
4.1
作者：
Bryantsev, Anton L.;Kurchashova, Svetlana Yu.;Kampinga, Harm H.
通讯作者：
Kampinga, Harm H.
影响因子：
3.6
作者：
WISE, GE;LIN, F;FAN, W
通讯作者：
FAN, W