Genetic Relationships in Zoysia Species and the Identification of Putative Interspecific Hybrids Using Simple Sequence Repeat Markers and Inflorescence Traits
Genetic Relationships in Zoysia Species and the Identification of Putative Interspecific Hybrids Using Simple Sequence Repeat Markers and Inflorescence Traits
复制标题
DOI：
10.2135/cropsci2012.04.0218
复制
发表时间：
2013-01-01
期刊：
影响因子：
2.3
通讯作者：
Milla-Lewis, Susana
中科院分区：
文献类型：
--
作者：
Kimball, Jennifer A.;Zuleta, M. Carolina;Milla-Lewis, Susana
结缕草是暖季型草坪草，由于其上级的耐热性和耐旱性以及相对较低的投入要求，在整个美国南部和向上进入过渡区使用。了解结缕草属种质的种群结构有助于植物育种者利用现有的变异。本研究的目的是评估结缕草属内和种间的简单重复序列（SSR）等位基因多样性，评估推定种间杂种的遗传构成，并确定结缕草属是否。杂种可通过花序性状进行区分。选择62份结缕草种质作为一个子集的基因型和表型多样的基因型与50个SSR标记和6个花序性状进行评价。两个未加权对组的方法与算术平均（UPGMA）聚类分析和主坐标分析（PCO）分析显示，一个连续的遗传变异存在于物种内和物种之间。基于模型的程序STRUCTURE揭示了Z中两个不同的亚群。japonica Steud.以及一个明显的Z matrella（L.）Merr.亚群根据SSR分析，23份供试结缕草属材料存在杂合现象。六花序表型也揭示了一个连续的变化范围。本研究能够验证Z. japonica和Z. matrella，进一步证实了结缕草属植物（Zoysia spp.）是一个物种内的亚群或生态型，而不是单独的物种。
Zoysia Willd. are warm-season turfgrasses used throughout the southern United States and upward into the transition zone for their superior heat and drought tolerances and their relatively low input requirements. Understanding the population structure present within Zoysia germplasm can assist plant breeders in exploiting available variation. The objectives of this study were to assess simple sequence repeat (SSR) allelic diversity within and among Zoysia spp., evaluate the genetic constitution of putative interspecific hybrids, and determine if Zoysia spp. and hybrids can be differentiated by inflorescence traits. Sixty-two Zoysia accessions selected as a subset of genotypically and phenotypically diverse genotypes were evaluated with 50 SSR markers and six inflorescence traits. Both an unweighted pair group method with arithmetic averaging (UPGMA) phylogram and principal coordinate analysis (PCO) analysis revealed a continuum of genetic variation present within and among the species. The model-based program STRUCTURE revealed two distinct subpopulations within Z. japonica Steud. as well as a distinct Z. matrella (L.) Merr. subpopulation. Based on SSR analysis, 23 Zoysia accessions evaluated had evidence of admixture. Six inflorescence phenotypes also revealed a continuous range of variation. This study was able to verify the presence of hybrids between Z. japonica and Z. matrella and provided further evidence for the hypothesis that Zoysia spp. are subpopulations or ecotypes within one species and not separate species.