Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli
Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli
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DOI：
10.1101/gad.366606
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发表时间：
2006-01-01
影响因子：
10.5
通讯作者：
Gerdes, K
中科院分区：
文献类型：
--
作者：
Kruse, T;Blagoev, B;Gerdes, K
肌动蛋白样MreB细胞骨架蛋白和RNA聚合酶（RNAP）都被认为是染色体分离的动力。在这里，我们确定MreB和RNAP在体内相互作用的合作伙伴。使用体外纯化的组分证实了相互作用。我们还提出了令人信服的证据，MreB和RNAP都需要在大肠杆菌染色体分离。MreB是起源和大量DNA分离所必需的，而RNAP是大量DNA、末端以及可能也是起源分离所必需的。此外，流式细胞术分析表明，MreB耗竭和RNAP的失活赋予几乎相同的和高度不寻常的染色体分离缺陷。因此，我们的研究结果提出了MreB-RNAP相互作用对染色体分离具有重要功能的可能性。
The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.