Trace determination of bisphenol A and phytoestrogens in infant formula powders by gas chromatography-mass spectrometry
Trace determination of bisphenol A and phytoestrogens in infant formula powders by gas chromatography-mass spectrometry
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DOI：
10.1016/j.chroma.2003.08.084
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发表时间：
2004-02-20
影响因子：
4.1
通讯作者：
Ding, WH
中科院分区：
文献类型：
--
作者：
Kuo, HW;Ding, WH
本研究描述了一种可靠和灵敏的方法，同时测定双酚A（BPA）和两个主要的植物雌激素，大豆黄酮和染料木素，在奶粉和婴儿配方奶粉的三甲基硅烷化后的气相色谱-质谱分析。为了减少与配方成分相关的基质干扰，首先将溶解的配方溶液超离心，然后使用C-18固相萃取柱提取上清液中的分析物。该方法的准确度和精密度进行了测定，并成功地采用该技术来测定痕量浓度的双酚A，大豆苷元和染料木素的配方粉。结果表明，BPA、大豆苷元和染料木黄酮在所有测试样品（n = 6）中检测到，浓度分别为45 - 113 ng/g（一种婴儿配方奶粉除外）、20 - 2050 ng/g和21 - 65 10 ng/g。大豆苷元和染料木素的最高浓度（即，2050和6510 ng/g）。定量限为1.0纳克/克双酚A，大豆苷元和染料木素10纳克/去使用0.5克奶粉样品。(C)2003 Elsevier B. V.保留所有权利。
This investigation describes a reliable and sensitive method for simultaneously determining bisphenol A (BPA) and two major phytoestrogens, daidzein and genistein, in powdered milks and infant formulas by gas chromatography-mass spectrometric analysis after trimethylsilylation. To reduce the matrix interference associated with the constituents of the formulas, the dissolved formula solutions were firstly ultra-centrifuged and the analytes in the supernatant were then extracted using a C-18 solid-phase extraction column. The accuracy and precision of the method were determined and the technique was successfully employed to measure trace concentrations of BPA, daidzein and,genistein in powdered formulas. The results show that BPA, daidzein and genistein were detected in all the testing samples (n = 6) at concentrations from 45 to 113 ng/g (except one infant formula), 20 to 2050 ng/g and 21 to 65 10 ng/g, respectively. The highest concentrations of daidzein and genistein (i.e., 2050 and 6510 ng/g) were detected in a soy-based powdered infant formula. The quantitation limits were 1.0 ng/g for BPA, and 10 ng/go for daidzein and genistein using 0.5 g powdered milk samples. (C) 2003 Elsevier B.V. All rights reserved.