Three-Dimensional Culture Systems and Humanized Liver Models Using Hepatic Stem Cells for Enhanced Toxicity Assessment
Three-Dimensional Culture Systems and Humanized Liver Models Using Hepatic Stem Cells for Enhanced Toxicity Assessment
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使用肝干细胞的三维培养系统和人源化肝脏模型增强毒性评估
DOI：
10.1002/9781119135449.ch8
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hideki Taniguchi
中科院分区：
文献类型：
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作者：
Ran-Ran Zhang;Yun-Wen Zheng;Hideki Taniguchi
原代人肝细胞和细胞系通常用于评估化学品的毒性。然而，由于胚胎干细胞和诱导多能干细胞衍生的肝细胞具有无限的扩增能力，因此在临床前研究中可能更具吸引力。将干细胞与肝脏多细胞协会的3D培养系统、人源化啮齿动物肝脏的体外系统和体内系统相结合，扩大应用范围，可以改善毒性和临床前研究。
Primary human hepatocytes and cell lines are commonly used to assess the toxicity of chemicals. However, embryonic stem cells and induced pluripotent stem cell‐derived hepatocytes are potentially more attractive for preclinical purposes, given their unlimited expansion capability. The expanded application of stem cells combined with 3D culture systems using a hepatic multiple‐cell society,ex vivosystems using humanized rodent livers, andin vivosystems could lead to improved toxicity and preclinical studies.