Infant B Cell Memory Differentiation and Early Gut Bacterial Colonization
Infant B Cell Memory Differentiation and Early Gut Bacterial Colonization
复制标题
DOI：
10.4049/jimmunol.1103223
复制
发表时间：
2012-05-01
影响因子：
4.4
通讯作者：
Rudin, Anna
中科院分区：
文献类型：
--
作者：
Lundell, Anna-Carin;Bjornsson, Viktor;Rudin, Anna
无菌动物模型表明，肠道的共生细菌定植可诱导B细胞分化和激活。在儿童早期，特定细菌种类或群体的定植是否与B细胞发育有关尚不清楚。在一项包括65名瑞典儿童的前瞻性新生儿/婴儿队列中，我们使用流式细胞术检测了在生命最初3岁的几个时间点获得的血液样本中CD20（+）、CD5（+）和CD27(+) B细胞的数量和比例。收集粪便样本，并在1、2、4和8周时对主要兼性和厌氧细菌进行定量培养。我们发现CD20(+) B细胞和CD5(+)CD20(+) B细胞的数量在4月龄时达到最高水平，而表达记忆标记CD27的CD20(+) B细胞在18月龄和36月龄时数量最多。通过多变量分析，我们发现大肠杆菌和双歧杆菌的早期定植与4月龄和18月龄时表达记忆标记CD27的CD20(+) B细胞数量较高相关。相反，我们无法证明细菌定植模式与CD20（+）或CD5(+)CD20(+) B细胞数量之间的任何关系。这些结果表明，肠道细菌定植模式也可能影响人类B细胞的成熟，包括大肠杆菌和双歧杆菌在内的早期肠道微生物群可能促进这种成熟。免疫学杂志，2012,18(8):415 - 422。
Germ-free animal models have demonstrated that commensal bacterial colonization of the intestine induces B cell differentiation and activation. Whether colonization with particular bacterial species or groups is associated with B cell development during early childhood is not known. In a prospective newborn/infant cohort including 65 Swedish children, we examined the numbers and proportions of CD20(+), CD5(+), and CD27(+) B cells in blood samples obtained at several time points during the first 3 y of life using flow cytometry. Fecal samples were collected and cultured quantitatively for major facultative and anaerobic bacteria at 1, 2, 4, and 8 wk of life. We found that the numbers of CD20(+) B cells and CD5(+)CD20(+) B cells reached their highest levels at 4 mo, whereas CD20(+) B cells expressing the memory marker CD27 were most numerous at 18 and 36 mo of age. Using multivariate analysis, we show that early colonization with Escherichia coli and bifidobacteria were associated with higher numbers of CD20(+) B cells that expressed the memory marker CD27 at 4 and 18 mo of age. In contrast, we were unable to demonstrate any relation between bacterial colonization pattern and numbers of CD20(+) or CD5(+)CD20(+) B cells. These results suggest that the intestinal bacterial colonization pattern may affect the B cell maturation also in humans, and that an early gut microbiota including E. coli and bifidobacteria might promote this maturation. The Journal of Immunology, 2012, 188: 4315-4322.