A university outbreak of gastroenteritis due to a small round-structured virus. Application of molecular diagnostics to identify the etiologic agent and patterns of transmission.
A university outbreak of gastroenteritis due to a small round-structured virus. Application of molecular diagnostics to identify the etiologic agent and patterns of transmission.
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大学爆发由小型圆形结构病毒引起的胃肠炎。
DOI：
10.1093/infdis/173.4.787
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
R. Glass
中科院分区：
文献类型：
--
作者：
P. Kilgore;E. Belay;Daniel M. Hamlin;J. Noel;C. Humphrey;H. Gary;T. Ando;S. Monroe;P. Kludt;D. S. Rosenthal;J. Freeman;R. Glass
对1994年12月肠胃炎爆发的流行病学调查表明，午餐时食用沙拉与2天的疾病有关（12月5日：优势比[OR]=3.1, 95%可信区间[CI]=2.0-5.0； 12月6日：OR=3.1, 95%可信区间[CI]= 1.9-4.9）。对患病学生和教职工（病例-患者）的单次粪便或呕吐物标本进行细菌和病毒病原体检测。电镜检查19例患者中9例的粪便标本和5例患者中3例的呕吐物标本中检出小圆结构病毒（SRSVs）。逆转录聚合酶链反应（RT-PCR）鉴定srsv为G-2/P2-B型菌株。患病学生呕吐物和粪便标本的RT-PCR产物的核苷酸序列与患病沙拉厨师粪便标本的核苷酸序列相同。这些发现表明，单一SRSV菌株是暴发的病原，可能通过食用受污染的沙拉传播给学生。流行病学调查与分子诊断相结合，可使早期发现传染源并改善疫情控制。
An epidemiologic investigation of a gastroenteritis outbreak in December 1994 indicated that salad consumption during lunch was linked with illness on 2 days (5 December: odds ratio [OR]=3.1, 95% confidence interval [CI]=2.0-5.0; 6 December: OR=3.1, 95% CI=1.9-4.9). Single stool or vomitus specimens from ill students and staff (case-patients) were examined for bacterial and viral pathogens. Small round-structured viruses (SRSVs) were detected by electron microscopy in stool specimens from 9 of 19 case-patients and in vomitus specimens from 3 of 5 case-patients. By reverse transcription-polymerase chain reaction (RT-PCR), the SRSVs were shown to be G-2/P2-B type strain. The nucleotide sequences of RT-PCR products from vomitus and stool specimens of ill students were identical to stool specimens from the ill salad chef. These findings suggest that a single SRSV strain was the etiologic agent in the outbreak that was possibly transmitted to students through consumption of contaminated salad. Epidemiologic investigation in conjunction with molecular diagnostics may enable early identification of sources of infection and improve outbreak control.