Shared characteristics underpinning C 4 leaf maturation derived from analysis of multiple C 3 and C 4 species of Flaveria
Shared characteristics underpinning C 4 leaf maturation derived from analysis of multiple C 3 and C 4 species of Flaveria
复制标题
通过对多种黄菊属 C 3 和 C 4 物种的分析得出支持 C 4 叶成熟的共同特征
DOI：
10.1101/088153
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kümpers B
中科院分区：
文献类型：
--
作者：
Kümpers B
Most terrestrial plants use C3photosynthesis to fix carbon. In multiple plant lineages a modified system known as C4photosynthesis has evolved. To better understand the molecular patterns associated with induction of C4photosynthesis, the genusFlaveriathat contains C3and C4species was used. A base to tip maturation gradient of leaf anatomy was defined, and RNA sequencing was undertaken along this gradient for two C3and two C4Flaveriaspecies. Key C4traits including vein density, mesophyll and bundle sheath cross-sectional area, chloroplast ultrastructure, and abundance of transcripts encoding proteins of C4photosynthesis were quantified. Candidate genes underlying each of these C4characteristics were identified. Principal components analysis indicated that leaf maturation and the photosynthetic pathway were responsible for the greatest amount of variation in transcript abundance. Photosynthesis genes were over-represented for a prolonged period in the C4species. Through comparison with publicly available data sets, we identify a small number of transcriptional regulators that have been up-regulated in diverse C4species. The analysis identifies similar patterns of expression in independent C4lineages and so indicates that the complex C4pathway is associated with parallel as well as convergent evolution.
