Mass imaging and identification of biomolecules with MALDI-QIT-TOF-based system
Mass imaging and identification of biomolecules with MALDI-QIT-TOF-based system
复制标题
DOI：
10.1021/ac071301v
复制
发表时间：
2008-02-01
影响因子：
7.4
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Shimma, Shuichi;Sugiura, Yuki;Setou, Mitsutoshi
成像质谱正在成为医学和生物科学中流行的可视化技术。对于其持续发展，使用串联质谱法（MSn）直接在组织表面上可视化和识别分子的能力是必不可少的。我们建立了一个基于基质辅助激光/解吸电离四极离子阱飞行时间型仪器（AXIMA-QIT，岛津，京都，日本），这是兼容的成像和高灵敏度MSn的成像系统。在本文中，我们提出的操作条件的AXIMA-QIT作为一种成像仪器，并介绍了我们开发的数据转换器是免费提供的。转换后的数据可以应用于常用的可视化软件Biomap。在可行性实验中，我们展示了小鼠小脑中磷脂、糖脂和胰蛋白酶消化的蛋白质的可视化。通过直接在组织表面上的MSn成功地识别了可视化的脂质，具有很强的分离前体离子的能力。在胰蛋白酶消化的蛋白质的分析中，我们比较了AXIMA-QIT和串联TOF型仪器之间的产物离子谱。结果证实，AXIMA-QIT即使在组织表面上也可以提供高质量的产物离子谱。
Imaging mass spectrometry is becoming a popular visualization technique in the medical and biological sciences. For its continued development, the ability to both visualize and identify molecules directly on the tissue surface using tandem mass spectrometry (MSn) is essential. We established an imaging system based on a matrix-assisted laser/desorption ionization quadrupole ion trap time-of-flight type instrument (AXIMA-QIT, Shimadzu, Kyoto, Japan), which was compatible with both imaging and highly sensitive MSn. In this paper, we present the operating conditions of the AXIMA-QIT as an imaging instrument and introduce the data converter we developed that is available free of charge. The converted data can be applied to Biomap, the commonly used visualization software. For the feasibility experiments, we demonstrated the visualization of phospholipids, glycolipid, and tryptic-digested proteins in the mouse cerebellum. The visualized lipids were successfully identified by MSn directly on the tissue surface, with a strong ability to isolate precursor ions. In the analysis of tryptic-digested proteins, we compared the product ion spectra between AXIMA-QIT and a tandem TOF-type instrument. The results confirmed that AXIMA-QIT can provide a high quality of product ion spectra even on the tissue surface.