Rps8, A New Locus in Soybean for Resistance to Phytophthora sojae
Rps8, A New Locus in Soybean for Resistance to Phytophthora sojae
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DOI：
10.2135/cropsci2003.1010
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发表时间：
2003
期刊：
影响因子：
2.3
通讯作者：
K. D. Burnham;A. Dorrance;D. Francis;R. Fioritto;S. S. Martin-S.
中科院分区：
文献类型：
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作者：
K. D. Burnham;A. Dorrance;D. Francis;R. Fioritto;S. S. Martin-S.
由大豆疫霉 (Phytophthora sojae) 引起的根腐病和茎腐病 M.J. Kaufmann & J.W. Gerdemann（=Phytophthora megasperma Drechs.f.sp.glycinea T.Kuan & D.C.Erwin）是美国大豆 [Glycine max (L.) Merr.] 产量损失的第二大原因。目前，已在大豆中鉴定出 7 个抗性位点，13 个等位基因。许多大豆产区都存在大豆疫霉种群，它们会导致带有许多（如果不是全部）这些基因的植物患病。因此，非常需要新的抗性基因座。一项早期研究发现，许多来自韩国的植物引进（PI）可能含有新的抗性位点。该项目的目标是鉴定 PI399073 中存在的抗酱油病等位基因，并将这些等位基因定位到连锁群。 PI399073 与“Williams”和“S 19-90”杂交以发展种群。对这些群体的抗病性、单序列重复（SSR）、限制性片段长度多态性（RFLP）和同工酶标记进行了分析。在与 PI399073 的两次不同杂交中，一个新的疫霉属抗性基因座 Rps8 被定位到 MLG（主要连锁群）A2。这是在 MLG A2 上发现的第一个疫霉抗性位点。
Phytophthora root and stem rot caused by Phytophthora sojae M.J. Kaufmann & J.W. Gerdemann (=Phytophthora megasperma Drechs. f. sp. glycinea T. Kuan & D.C. Erwin) is the second leading cause of yield loss in soybean [Glycine max (L.) Merr.] in the USA. Currently, seven loci for resistance with 13 alleles have been identified in soybean. Populations of P. sojae exist in many soybean production regions that cause disease on plants with many, if not all, of these genes. Consequently, the need for new resistance loci is great. A number of plant introductions (PIs) from South Korea were identified in an earlier study that may contain novel resistance loci. The objectives of this project were to identify the allele(s) for resistance to P. sojae present in PI399073 and to map these allele(s) to a linkage group. PI399073 was crossed with ‘Williams’ and ‘S 19-90’ to develop populations. These populations were assayed for disease resistance, single sequence repeats (SSR), restriction fragment length polymorphisms (RFLP), and isozyme markers. A new Phytophthora resistance locus, Rps8, was mapped to MLG (major linkage group) A2 in two different crosses with PI399073. This is the first locus for Phytophthora resistance that has been identified on MLG A2.