Proliferative responses of mononuclear cells in Lyme disease. Reactivity to Borrelia burgdorferi antigens is greater in joint fluid than in blood.
Proliferative responses of mononuclear cells in Lyme disease. Reactivity to Borrelia burgdorferi antigens is greater in joint fluid than in blood.
复制标题
莱姆病中单核细胞的增殖反应。
DOI：
10.1002/art.1780290609
复制
发表时间：
1986
影响因子：
--
通讯作者：
Dwyer,JM
中科院分区：
文献类型：
--
作者：
Sigal,LH;Steere,AC;Freeman,DH;Dwyer,JM
在27名早期莱姆病患者中，外周血单核细胞（PBMC）对莱姆病螺旋体伯氏疏螺旋体抗原的平均反应（每分钟723个计数）与对照受试者相似。在2-3周后的恢复期，患者的平均反应显著更高（2，075 cpm，P< 0.008）。22例莱姆病患者的PBMC对伯氏疏螺旋体的反应比早期患者更强（2,923 cpm，P< 0.0004），而同时获得的滑液单个核细胞的反应最强（15,238 cpm，P< 0.001）。早期莱姆病患者的PBMC反应略低于植物血凝素和美洲商陆有丝分裂原比正常对照组，但关节炎患者有大于正常的有丝分裂原的反应。与此相反，滑液细胞间的丝裂原反应性显着降低，并与抗原反应呈负相关。因此，在莱姆病患者中，单核细胞的抗原特异性反应随着疾病的进展而增加，而在关节炎患者中，对抗原的最大反应性见于炎症关节中的细胞。
In 27 patients with early Lyme disease, the mean response of peripheral blood mononuclear cells (PBMC) to Lyme spirochetalBorrelia burgdorferiantigens (723 counts per minute) was similar to that of control subjects. During convalescence, 2–3 weeks later, the patients' mean response was significantly higher (2,075 cpm,P< 0.008). Compared with those with early disease, the PBMC of 22 patients with Lyme arthritis reacted even more toB burgdorferi(2,923 cpm,P< 0.0004), and, by far, the greatest response was in concomitantly obtained synovial fluid mononuclear cells (15,238 cpm,P< 0.001). The PBMC of patients with early Lyme disease reacted slightly less to phytohemagglutinin and pokeweed mitogen than those of normal control subjects, but patients with arthritis had greater than normal mitogen responses. In contrast, mitogen reactivity among synovial fluid cells was markedly decreased and correlated inversely with the response to antigen. Thus, in patients with Lyme disease, the antigen‐specific responses of mononuclear cells increase as the disease progresses, and in those with arthritis, the greatest reactivity to antigen is found in cells in the inflamed joint.