Cell expansion and endoreduplication show a large genetic variability in pericarp and contribute strongly to tomato fruit growth
Cell expansion and endoreduplication show a large genetic variability in pericarp and contribute strongly to tomato fruit growth
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DOI：
10.1104/pp.105.068767
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发表时间：
2005-12-01
期刊：
影响因子：
7.4
通讯作者：
Renaudin, JP
中科院分区：
文献类型：
--
作者：
Cheniclet, C;Rong, WY;Renaudin, JP
番茄（Solanum lycopersicon）和许多类型的水果一样，花后生长依赖于细胞分裂和细胞扩增，因此在植物中发现的一些最大的细胞出现在肉质果实中。已知核内复制发生在这样的材料中，这表明其参与细胞扩增，尽管还没有数据证明这一假设。我们分析了番茄果皮的图案形成、细胞大小和倍性。第一组数据是在整个果实发育过程中在一个樱桃番茄品系中收集的。第二组数据是从20个番茄品系中获得的，这些番茄品系在果实中显示出大的重量范围，将其作为开花期的子房和作为破花期的完全生长的果实进行比较。在开花期，所有20个番茄品系的果皮形态（包括细胞层数和细胞大小）都具有显著的保守性，而开花后的生长变化较大。结合发育和遗传多样性，在平均细胞大小和倍性之间证明了强的正相关，这适用于平均细胞直径从10至350 mm（即32，000倍体积变化）和平均倍性水平从3至80 ℃。果实重量与细胞大小和倍性也呈显著相关。这些数据提供了果皮图案化和生长的框架。他们强烈建议多倍体相关的细胞扩增作为番茄果实重量的决定因素的数量重要性。
Postanthesis growth of tomato (Solanum lycopersicon) as of many types of fruit relies on cell division and cell expansion, so that some of the largest cells to be found in plants occur in fleshy fruit. Endoreduplication is known to occur in such materials, which suggests its involvement in cell expansion, although no data have demonstrated this hypothesis as yet. We have analyzed pattern formation, cell size, and ploidy in tomato fruit pericarp. A first set of data was collected in one cherry tomato line throughout fruit development. A second set of data was obtained from 20 tomato lines displaying a large weight range in fruit, which were compared as ovaries at anthesis and as fully grown fruit at breaker stage. A remarkable conservation of pericarp pattern, including cell layer number and cell size, is observed in all of the 20 tomato lines at anthesis, whereas large variations of growth occur afterward. A strong, positive correlation, combining development and genetic diversity, is demonstrated between mean cell size and ploidy, which holds for mean cell diameters from 10 to 350 mm (i.e. a 32,000-times volume variation) and for mean ploidy levels from 3 to 80 C. Fruit weight appears also significantly correlated with cell size and ploidy. These data provide a framework of pericarp patterning and growth. They strongly suggest the quantitative importance of polyploidy-associated cell expansion as a determinant of fruit weight in tomato.