PRESENCE OF ENV GENES IN MEMBERS OF THE RTVL-H FAMILY OF HUMAN ENDOGENOUS RETROVIRUS-LIKE ELEMENTS
PRESENCE OF ENV GENES IN MEMBERS OF THE RTVL-H FAMILY OF HUMAN ENDOGENOUS RETROVIRUS-LIKE ELEMENTS
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DOI：
10.1006/viro.1993.1007
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发表时间：
1993-01-01
期刊：
影响因子：
3.7
通讯作者：
HARADA, F
中科院分区：
文献类型：
--
作者：
HIROSE, Y;TAKAMATSU, M;HARADA, F
我们已经发现RTVL-H家族的人内源性逆转录病毒样元件在肺鳞状细胞癌组织和几种人细胞系中高度表达。我们确定了几个cDNA克隆的核苷酸序列，并发现了一个独特的序列，这是不存在于已公布的“原型”RTVL-H序列。该区域中的开放阅读框编码与各种逆转录病毒的跨膜包膜蛋白中的保守序列同源的氨基酸序列。获得编码该序列的两个基因组克隆并测序。一个含有env基因的RTVL-H元件长度为8.7个酶对。推定的env基因，由约1800个碱基对组成，有两个开放的阅读框架中断的终止密码子。该区域的氨基酸序列与其他逆转录病毒包膜蛋白的氨基酸序列具有显著的同源性，并含有8个潜在的糖基化位点。估计每个单倍体人基因组中含有约100个拷贝的含有env基因的RTVL-H元件。
We have found that the RTVL-H family of human endogenous retrovirus-like elements was highly expressed in lung squamous cell carcinoma tissue and several human cell lines. We determined the nucleotide sequences of several cDNA clones and found a unique sequence that was not present in the published "prototypic" RTVL-H sequence. An open reading frame in this region encoded an amino acid sequence homologous to the conserved sequence in the transmembrane envelope protein of various retroviruses. Two genomic clones that encoded this sequence were obtained and sequenced. One RTVL-H element containing the env gene was 8.7 kilobase pairs in length. The putative env gene, consisting of about 1800 base pairs, had two open reading frames interrupted by a termination codon. The amino acid sequence of this region showed significant homology with these of other retroviral envelope proteins and contained eight potential glycosylation sites. There were estimated to be about 100 copies of RTVL-H elements containing the env gene per haploid human genome.