Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model
Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model
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DOI：
10.4236/ojrm.2013.21002
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发表时间：
2013-03
期刊：
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通讯作者：
Kang Chen;J. Hao;Kanako Noritake;Y. Yamashita;S. Kuroda;S. Kasugai
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文献类型：
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作者：
Kang Chen;J. Hao;Kanako Noritake;Y. Yamashita;S. Kuroda;S. Kasugai
目的：低强度脉冲超声刺激（LIPUS）已在骨科领域临床应用于促进骨折愈合。因此，LIPUS 也可能刺激颅颌面区域骨缺损的骨再生。然而，这一点尚未得到明确证明。此外，手术后应用的最佳时间点和时期尚未报道。本研究的目的是评估LIPUS对大鼠顶骨缺损骨再生的影响，特别关注应用的时间和周期。材料和方法：18只Wistar大鼠（14周龄）分为6组：5个实验组和1个对照组。在顶骨两侧制备直径 5 mm 的骨缺损，并将定制的明胶膜放置在骨缺损上。根据应用时间表，在实验组中将 LIPUS（160 mW/cm2，15 分钟/天）应用于外部有源换能器的缺损区域：第 1 组（第 6 - 12 天）、第 2 组（第 13 - 19 天）、第 3 组（第 20 - 26 天）、第 4 组（第 6 - 19 天）和第 5 组（第 6 - 26 天）。所有动物在28天时被处死。通过显微 CT 分析缺陷，然后进行组织学分析。结果：第1组与对照组相比，新骨形成明显促进，新骨较厚且成熟。在其他实验组中，有刺激新骨形成的倾向；然而，它们在统计上并不显着。讨论和结论：本研究表明骨缺损处新骨形成的量取决于LIPUS应用的时间和周期。有人建议，在早期愈合期（术后第二周）应用 LIPUS 可有效加速新骨形成。
Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm2, 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 - 12), group 2 (day 13 - 19), group 3 (day 20 - 26), group 4 (day 6 - 19) and group 5 (day 6 - 26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.