Reliable Assessment of Swine Renal Fibrosis Using Quantitative Magnetization Transfer Imaging.
Reliable Assessment of Swine Renal Fibrosis Using Quantitative Magnetization Transfer Imaging.
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DOI：
10.1097/rli.0000000000000843
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发表时间：
2022-05-01
影响因子：
6.7
通讯作者：
Lerman LO
中科院分区：
文献类型：
--
作者：
Jiang K;Ferguson CM;Grimm RC;Zhu X;Glockner JF;Lerman LO
定量磁化转移 (qMT) 可用于测量高场强和超高场强下的小鼠肾纤维化。然而，它在临床场强和类人肾脏中的效用仍然未知。我们测试了 qMT 在 3.0T 下能够成功检测单侧肾动脉狭窄 (RAS) 猪肾脏纤维化的假设。 qMT 协议由具有可变翻转角和偏移频率的 MT 扫描以及 B0、B1 和 T1 映射组成。 RAS 或对照后 10 周对猪进行扫描。使用两池模型来拟合肾皮质 (CO) 和外髓质 (OM) 的结合池分数 f。然后将 5 头正常猪和 10 头 RAS 猪的 qMT 衍生 f 与使用 Masson 三色染色确定的组织学纤维化以及使用计算机断层扫描评估的肾灌注进行比较。 qMT 双池模型提供了猪肾脏收集数据的准确拟合。与正常肾脏相比，狭窄肾脏的 CO（9.8±2.7% vs. 6.4±0.9%，P=0.002）和 OM（7.6±2.2% vs. 4.7±1.1%，P=0.002）f 均显着升高。组织学测量的肾纤维化和 qMT 衍生的 f 在皮质（Pearson 相关系数 r=0.93，P<0.001）和 OM（r=0.84，P=0.002）中直接相关，与狭窄肾灌注呈反比（r=0.85，P=0.002）。本研究证明了 qMT 用于测量类人猪肾脏纤维化的可行性，以及组织大分子含量与肾脏灌注之间的关联。因此，qMT 可能可作为临床领域实力的 RAS 受试者肾纤维化无创评估工具。
Quantitative magnetization transfer (qMT) is useful for measurement of murine renal fibrosis at high and ultrahigh field-strengths. However, its utility at clinical field-strengths and in human-like kidneys remains unknown. We tested the hypothesis that qMT would successfully detect fibrosis in swine kidneys with unilateral renal artery stenosis (RAS) at 3.0T. The qMT protocol comprised of MT scans with variable flip angles and offset frequencies, and of B0, B1, and T1 mapping. Pigs were scanned 10 weeks after RAS or control. A two-pool model was used to fit the bound pool fraction f of the renal cortex (CO) and outer medulla (OM). Then qMT-derived f in 5 normal and 10 RAS pigs was compared to histological fibrosis determined using Masson’s trichrome staining and to renal perfusion assessed with computed-tomography. The qMT two-pool model provided accurate fittings of data collected on swine kidneys. Stenotic kidneys showed significantly elevated f in both the CO (9.8±2.7% vs. 6.4±0.9%, P=0.002) and OM (7.6±2.2% vs. 4.7±1.1%, P=0.002), as compared to normal kidneys. Histology-measured renal fibrosis and qMT-derived f correlated directly in both the cortex (Pearson’s correlation coefficient r=0.93, P<0.001) and OM (r=0.84, P=0.002), and inversely with stenotic kidney perfusion (r=0.85, P=0.002). This study demonstrates the feasibility of qMT for measuring fibrosis in human-like swine kidneys, and the association between tissue macromolecule content and renal perfusion. Therefore, qMT may be useful as a tool for noninvasive assessment of renal fibrosis in subjects with RAS at clinical field strengths.