Slow Development Restores the Fertility of Photoperiod-Sensitive Male-Sterile Plant Lines1[OPEN]
Slow Development Restores the Fertility of Photoperiod-Sensitive Male-Sterile Plant Lines1[OPEN]
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DOI：
10.1104/pp.20.00951
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发表时间：
2020-08
期刊：
影响因子：
7.4
通讯作者：
Cheng Zhang;Te Xu;Meng-Yi Ren;Jun Zhu;Qiangsheng Shi;Ya-Fei Zhang;Yi-Wen Qi;Min-Jia Huang;Lei Song;Ping Xu;Zhongnan Yang
中科院分区：
文献类型：
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作者：
Cheng Zhang;Te Xu;Meng-Yi Ren;Jun Zhu;Qiangsheng Shi;Ya-Fei Zhang;Yi-Wen Qi;Min-Jia Huang;Lei Song;Ping Xu;Zhongnan Yang
发育迟缓是光敏核不育的普遍机制。光温敏核不育系在作物育种中有着广泛的应用。拟南芥（Arabidopsis thaliana）温敏核不育系（包括胼胝质壁形成缺陷的cals 5 -2）的育性转换依赖于低温下的缓慢发育。在本研究中，我们发现cals 5 -2也表现出PGMS。在短日照或低光照条件下，花粉发育缓慢的cals 5 -2育性恢复，表明在这些条件下，缓慢的发育恢复cals 5 - 2的育性。我们发现，其他几个花粉壁形成缺陷的温敏核不育系也表现出光敏核不育特性。这种相似性表明，缓慢发育是光敏核不育育性恢复的一般机制。值得注意的是，缓慢的发育也是温敏核不育系育性恢复的基础。进一步的分析揭示了这些P/TGMS系在允许条件下功能花粉形成过程中的花粉壁特征。我们的结论是，缓慢的发展是一个普遍的机制，P/TGMS系的育性恢复，并允许这些植物采取不同的策略，以克服花粉形成缺陷。
Slow development is a general mechanism for photoperiod-sensitive genic male sterility. Photoperiod- and thermosensitive genic male sterility (P/TGMS) lines are widely used in crop breeding. The fertility conversion of Arabidopsis (Arabidopsis thaliana) TGMS lines including cals5-2, which is defective in callose wall formation, relies on slow development under low temperatures. In this study, we discovered that cals5-2 also exhibits PGMS. Fertility of cals5-2 was restored when pollen development was slowed under short-day photoperiods or low light intensity, suggesting that slow development restores the fertility of cals5-2 under these conditions. We found that several other TGMS lines with defects in pollen wall formation also exhibited PGMS characteristics. This similarity indicates that slow development is a general mechanism of PGMS fertility restoration. Notably, slow development also underlies the fertility recovery of TGMS lines. Further analysis revealed the pollen wall features during the formation of functional pollens of these P/TGMS lines under permissive conditions. We conclude that slow development is a general mechanism for fertility restoration of P/TGMS lines and allows these plants to take different strategies to overcome pollen formation defects.