H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.
H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.
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H-2Kk 和水泡性口炎病毒 G 蛋白在 T 细胞识别的重建膜中并不广泛相关。
DOI：
10.1073/pnas.79.5.1506
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发表时间：
1982
影响因子：
11.1
通讯作者：
McConnell,HM
中科院分区：
文献类型：
--
作者：
Cartwright,GS;Smith,LM;Heinzelmann,EW;Ruebush,MJ;Parce,JW;McConnell,HM
结果表明，含有（i）荧光素标记的鼠组织相容性抗原（FITC-H-2Kk）和水泡性口炎病毒的G蛋白或（ii）H-2Kk和荧光素标记的病毒蛋白（FITC-G）的脂质体可以引发H-2限制的同系抗病毒细胞毒性T细胞，如从适当的病毒感染的靶细胞中释放的51 Cr所测定的。使用光漂白后的荧光恢复来测量这些重构蛋白在四种不同样品中的扩散系数：（i）FITC-H-2Kk;（ii）FITC-H-2Kk和G;（iii）FITC-G;以及（iv）FITC-G和H-2Kk。在每种情况下都获得了相同的侧向扩散速率（在37 ℃下，在25%胆固醇/75%二肉豆蔻酰磷脂酰胆碱中D = 1 × 10（-8）cm 2/sec）。这两种蛋白质，无论是荧光蛋白还是非荧光蛋白，都可以通过使用特定抗体来修补。当G与抗体结合时，FITC-H-2Kk不能与G结合。当H-2Kk与抗体斑贴时，FITC-G不能与H-2Kk斑贴。这些扩散和修补测量排除了这些蛋白质具有广泛的寡聚缔合或强特异性成对缔合的可能性。
It is shown that liposomes containing (i) a fluorescein-labeled murine histocompatibility antigen (FITC-H-2Kk) and the G protein of vesicular stomatitis virus or (ii) H-2Kk and fluorescein-labeled viral protein (FITC-G) can elicit H-2-restricted syngeneic antiviral cytotoxic T cells as assayed by 51Cr release from appropriate virus-infected target cells. Fluorescence recovery after photobleaching was used to measure the diffusion coefficients of these reconstituted proteins in four different samples: (i) FITC-H-2Kk; (ii) FITC-H-2Kk and G; (iii) FITC-G; and (iv) FITC-G and H-2Kk. The same rate of lateral diffusion (D = 1 x 10(-8) cm2/sec at 37 degrees C in 25% cholesterol/75% dimyristoylphosphatidylcholine) was obtained in every case. Both proteins, fluorescent as well as nonfluorescent, could be patched by using specific antibodies. When G was patched with antibody, FITC-H-2Kk did not copatch. When H-2Kk was patched with antibody FITC-G did not copatch. These diffusion and patching measurements rule out the possibility that these proteins have either extensive oligomeric associations or strong specific pairwise associations.