The Cytotoxicity of Layered Black Phosphorus
The Cytotoxicity of Layered Black Phosphorus
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DOI：
10.1002/chem.201502006
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发表时间：
2015-09-28
影响因子：
4.3
通讯作者：
Pumera, Martin
中科院分区：
文献类型：
--
作者：
Latiff, Naziah Mohamad;Teo, Wei Zhe;Pumera, Martin
黑磷（BP）是二维层状材料家族的最新成员，由于潜在的光电子学、纳米电子学和生物医学应用而引起了人们的广泛兴趣。人们对其毒性知之甚少，例如它是否与白磷一样有毒。针对BP应用于商业产品和生物医学设备的可能性，研究了其对人肺癌上皮细胞（A549）的细胞毒性。将细胞暴露于不同 BP 浓度 24 小时后，使用水溶性四唑盐 (WST-8) 和甲基噻唑基二苯基溴化四唑 (MTT) 测定法进行细胞活力评估。发现毒理学效应具有剂量依赖性，在 50 μg mL(-1) 1 暴露下，BP 将细胞活力降低至 48% (WST-8) 和 34% (MTT)。据观察，这种毒性通常介于氧化石墨烯和剥离的过渡金属二硫属化物（MoS2、WS2、WSe2）之间。相对较低的毒性为黑磷的利用铺平了道路。
Black phosphorus (BP), the latest addition to the family of 2D layered materials, has attracted much interest owing to potential optoelectronics, nanoelectronics, and biomedicine applications. Little is known about its toxicity, such as whether it could be as toxic as white phosphorus. In response to the possibility of BP employment into commercial products and biomedical devices, its cytotoxicity to human lung carcinoma epithelial cells (A549) was investigated. Following a 24 h exposure of the cells with different BP concentrations, cell viability assessments were conducted using water-soluble tetrazolium salt (WST-8) and methylthiazolyldi-phenyltetrazolium bromide (MTT) assays. The toxicological effects were found to be dose-dependent, with BP reducing cell viabilities to 48% (WST-8) and 34% (MTT) at 50 mu g mL(-1) 1 exposure. This toxicity was observed to be generally intermediate between that of graphene oxides and exfoliated transition-metal dichalcogenides (MoS2, WS2, WSe2). The relatively low toxicity paves the way to utilization of black phosphorus.