Repair of canine mandibular bone defects with bone marrow stromal cells and porous β-tricalcium phosphate
Repair of canine mandibular bone defects with bone marrow stromal cells and porous β-tricalcium phosphate
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DOI：
10.1016/j.biomaterials.2006.10.015
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Yuan, Jie;Cui, Lei;Cao, Yilin
组织工程已成为骨缺损修复的新途径。以往的研究仅限于使用缓慢降解的支架与骨髓基质细胞（BMSCs）在下颌骨重建。在这项研究中，30 mm长的下颌骨节段性缺损的修复工程骨移植使用成骨诱导的自体骨髓基质细胞种植在多孔β-磷酸三钙（β-TCP，n = 5）。将缺损修复情况与单纯β-TCP（n = 6）或自体下颌骨段（n = 4）进行比较。BMSCs/beta-TCP组术后4周开始有新骨形成，32周后骨愈合，X线片和组织学检查显示骨愈合。相比之下，在单独使用β-TCP治疗的组中观察到几乎具有纤维连接的最小骨形成。术后32周，BMSCs/beta-TCP工程骨在弯曲载荷强度、弯曲位移、弯曲应力和弹性模量等方面均达到了较好的生物力学性能，与对侧无牙下颌骨和自体骨的生物力学性能接近（p > 0.05）。基于这些结果，我们得出结论，由成骨诱导的BMSCs和可生物降解的β-TCP组成的工程化骨可以很好地修复犬下颌骨临界尺寸的节段性缺损。(c)2006爱思唯尔有限公司保留所有权利。
Tissue engineering has become a new approach for repairing bone defects. Previous studies have been limited to the use of slow-degradable scaffolds with bone marrow stromal cells (BMSCs) in mandibular reconstruction. In this study, a 30 mm long mandibular segmental defect was repaired by engineered bone graft using osteogenically induced autologouse BMSCs seeded on porous beta-tricalcium phosphate (beta-TCP, n = 5). The repair of defects was compared with those treated with beta-TCP alone (n = 6) or with autologous mandibular segment (n = 4). In the BMSCs/beta-TCP group, new bone formation was observed from 4 weeks post-operation, and bony-union was achieved after 32 weeks, which was detected by radiographic and histological examination. In contrast, minimal bone formation with almost fibrous connection was observed in the group treated with beta-TCP alone. More importantly, the engineered bone with BMSCs/beta-TCP achieved a satisfactory biomechanical property in terms of bending load strength, bending displacement, bending stress and Young's modulus at 32 weeks post-operation, which was very close to those of contralateral edentulous mandible and autograft bone (p > 0.05). Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and biodegradable beta-TCP can well repair the critical-sized segmental mandibular defects in canines. (c) 2006 Elsevier Ltd. All rights reserved.