Vitamin D Inhibits Activities of Metalloproteinase-9/-13 in Articular Cartilage In Vivo and In Vitro
Vitamin D Inhibits Activities of Metalloproteinase-9/-13 in Articular Cartilage In Vivo and In Vitro
复制标题
维生素 D 在体内和体外抑制关节软骨中金属蛋白酶-9/-13 的活性
DOI：
10.3177/jnsv.65.107
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发表时间：
2019-01-01
影响因子：
1.6
通讯作者：
Leng, Huijie
中科院分区：
文献类型：
--
作者：
Li, Siwei;Niu, Guodong;Leng, Huijie
Low levels of serum vitamin D have been shown to accelerate progression of osteoarthritis. However, the role of vitamin D in articular cartilage degradation and osteoarthritis development is still unclear. This study investigated the effects of vitamin D on articular cartilage degradation by testing matrix metalloproteinase (MMPs) activities in articular cartilage using the rat vitamin D deficiency model at the animal level and rat articular chondrocytes at the cell level. The in vivo study showed vitamin D deficiency increased the expressions of MMP-9 and MMP-13 in rat articular cartilage, and the increase was inhibited by 1 alpha,25(OH)(2)D-3 supplementation. The increased production of MMP-9 and MMP-13 in the articular chondrocytes induced by tumor necrosis factor-alpha (TNF-alpha) or phorbol-12-myristate-13-acetate (PMA) was significantly suppressed by concomitant treatment with 1 alpha,25(OH)(2)D-3 in vitro. The increased level of C-telopeptide of type II collagen (CTX-II) induced by TNF-alpha or PMA was also significantly suppressed by concomitant treatment with 1 alpha,25(OH)(2)D-3 in vitro. Thus, vitamin D intake may inhibit MMP activities and take part in the process of articular cartilage degeneration and osteoarthritis progression.
