Multigaps Embedded Nanoassemblies Enhance In Situ Raman Spectroscopy for Intracellular Telomerase Activity Sensing
Multigaps Embedded Nanoassemblies Enhance In Situ Raman Spectroscopy for Intracellular Telomerase Activity Sensing
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DOI：
10.1002/adfm.201504587
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发表时间：
2016-03
影响因子：
19
通讯作者：
Liguang Xu;Sen Zhao;Wei Ma;Xiaoling Wu;Si-yuan Li;Hua Kuang;Libing Wang;Chuanlai Xu
中科院分区：
文献类型：
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作者：
Liguang Xu;Sen Zhao;Wei Ma;Xiaoling Wu;Si-yuan Li;Hua Kuang;Libing Wang;Chuanlai Xu
细胞内端粒酶的高灵敏度和定量生物检测是具有挑战性的。报道了一种编码拉曼报告基因（Cy 5）的DNA驱动的纳米颗粒自组装金字塔，其检测活细胞中的端粒酶。在存在靶点的情况下，端粒酶引物被延伸，内部DNA链被替换，导致表面增强拉曼散射（Sers）信号的降低和荧光信号的同时恢复。Sers信号在1 × 10-14 ~ 5 × 10-11 IU范围内与端粒酶活性呈良好的线性关系，检测限为6.2 × 10-15 IU。荧光信号用于确认细胞内端粒酶活性，证明所设计的金字塔探针的功效。这种生物传感策略为活细胞中生物标志物的定量提供了可靠且超灵敏的方案。
The highly sensitive and quantitative biodetection of intracellular telomerase is challenging. A DNA‐driven nanoparticle self‐assembling pyramid encoding a Raman reporter (Cy5) is reported that detects telomerase in live cells. In the presence of the target, the telomerase primer is extended and the inner DNA chain is replaced, leading to the reduction in the surface‐enhanced Raman scattering (SERS) signal and the simultaneous recovery of the fluorescent signal. The SERS signal has a linear range for the detection of telomerase in situ of 1 × 10–14 to 5 × 10–11 IU, with a limit of detection of 6.2 × 10–15 IU. The fluorescent signal is used to confirm the intracellular telomerase activity, demonstrating the efficacy of the designed pyramid probe. This biosensing strategy provides a reliable and ultrasensitive protocol for the quantification of biomarkers in living cells.