Molecular cloning and expression of a turgor-responsive gene in Brassica napus
Molecular cloning and expression of a turgor-responsive gene in Brassica napus
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DOI：
10.1007/bf00019320
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发表时间：
1995-02
影响因子：
5.1
通讯作者：
V. L. Stroeher;J. Boothe;A. Good
中科院分区：
文献类型：
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作者：
V. L. Stroeher;J. Boothe;A. Good
在干旱期间，植物激活了一些与适应水分胁迫有关的基因。我们已经从甘蓝型油菜中分离出一个这样的基因btg-26。btg-26在断水72h内诱导在叶片组织中表达。在81%的相对含水量（RWC）下，当植物刚刚开始表现出枯萎的迹象时，表达量已经比完全水化植物的叶组织增加了六倍。btg-26的表达在63% RWC时达到最大的11倍诱导，然后随着RWC的继续下降，转录物水平下降。btg-26也在高盐、低温、热休克和植物激素脱落酸的植物中被激活。对推导出的氨基酸序列进行分析，发现与脱氢酶家族相似。这些结果表明，btg-26编码一种蛋白质，其功能可能在一般渗透胁迫的早期需要一些未知的适应性代谢途径。
During droughting plants activate a number of genes involved in adaptation to water stress. We have isolated one such gene,btg-26, fromBrassica napus. Expression ofbtg-26is induced in leaf tissue within 72 h of withholding water. At 81% relative water content (RWC), when the plant is just beginning to show signs of wilting, expression is already increased six-fold over levels found in leaf tissue from fully hydrated plants.btg-26expression reaches a maximum eleven-fold induction at 63% RWC, then transcript levels decrease as RWC continues to drop.btg-26is also activated in plants exposed to high salinity, low temperature, heat shock and the plant hormone abscisic acid. Analysis of the deduced amino acid sequence revealed similarity to the dehydrogenase family of enzymes. These results suggest thatbtg-26encodes a protein whose function may be required early during general osmotic stress in some unknown adaptive metabolic pathway.