The hepatitis C virus 3'-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase.
The hepatitis C virus 3'-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase.
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DOI：
10.1093/nar/gkl019
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发表时间：
2006
影响因子：
14.9
通讯作者：
Gromeier, M
中科院分区：
文献类型：
--
作者：
Bradrick, SS;Walters, RW;Gromeier, M
真核信使RNA （mRNA）翻译起始的增强是通过poly(A)结合蛋白与真核起始因子（eIF） 4G的相互作用介导的，从而桥接5 '端帽结构。与细胞mRNA相反，未加帽、非多聚腺苷化的丙型肝炎病毒（HCV）基因组的翻译独立于eIF4G发生，3 ' -未翻译序列在修饰HCV基因表达中的作用存在争议。利用基于细胞和体外的翻译实验，我们发现HCV 3 ' -非翻译区（UTR）或足够长度的3 ' poly(a)通道交替刺激翻译依赖于HCV内部核糖体进入位点（IRES）。然而，与帽依赖翻译相反，HCV IRES的起始率不受3 ' -未翻译序列的影响。起始后事件的分析表明，3 ' poly(A) tract和HCV 3 ' -UTR通过使终止和可能的核糖体循环用于连续几轮翻译提高了翻译效率。
Enhancement of eukaryotic messenger RNA (mRNA) translation initiation by the 3′ poly(A) tail is mediated through interaction of poly(A)-binding protein with eukaryotic initiation factor (eIF) 4G, bridging the 5′ terminal cap structure. In contrast to cellular mRNA, translation of the uncapped, non-polyadenylated hepatitis C virus (HCV) genome occurs independently of eIF4G and a role for 3′-untranslated sequences in modifying HCV gene expression is controversial. Utilizing cell-based and in vitro translation assays, we show that the HCV 3′-untranslated region (UTR) or a 3′ poly(A) tract of sufficient length interchangeably stimulate translation dependent upon the HCV internal ribosomal entry site (IRES). However, in contrast to cap-dependent translation, the rate of initiation at the HCV IRES was unaffected by 3′-untranslated sequences. Analysis of post-initiation events revealed that the 3′ poly(A) tract and HCV 3′-UTR improve translation efficiency by enabling termination and possibly ribosome recycling for successive rounds of translation.
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