A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.
A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.
复制标题
一种针对分化抗原的单克隆抗体，存在于成熟人巨噬细胞上，但单核细胞中不存在。
作者：
G. Zwadlo;E. Bröcker;D. B. V. Bassewitz;U. Feige;C. Sorg
描述了一种在小鼠体内产生的针对培养的人血单核细胞/巨噬细胞的单抗。该抗体命名为25F9，属于IgG1亚类，可检测M.W.86,000，与新鲜分离的单核细胞不反应，但在培养3天后与单核细胞反应。巨噬细胞表面25F9抗原的表达随着培养时间的延长而增加。此外，该抗体对血小板、粒细胞、淋巴细胞和除两个黑色素瘤细胞系MeWo和MEL 57之外的大量人类细胞株均为阴性。在正常人体组织(皮肤、肺、肝脏、胸腺)以及炎性或肿瘤性组织(皮肤淋巴瘤、湿疹、卡介苗肉芽肿和黑色素瘤)的冰冻切片中，抗体与真皮中散布的巨噬细胞反应，但不与表皮朗格汉斯细胞、肺泡巨噬细胞、肝脏枯否细胞以及胸腺皮质和髓质中散布的巨噬细胞反应。在湿疹、卡介苗肉芽肿和皮肤淋巴瘤中，仅有少量浸润性巨噬细胞染色。另一方面，黑色素瘤中大量巨噬细胞和黑素噬细胞呈阳性反应。在某些情况下，黑色素瘤细胞也呈弱阳性。因此，该抗体检测到一种分化抗原，该抗原优先表达在成熟的组织固定的巨噬细胞上，而不存在于血液单核细胞中。
A monoclonal antibody is described that has been generated in the mouse against cultured human blood monocytes/macrophages. The antibody, designated 25F9, belongs to the IgG1 subclass, detects antigens of m.w. 86,000, and does not react with freshly isolated blood monocytes but reacts with monocytes after 3 days of culture. The expression of the 25F9 antigen on macrophages increases with culture time. Furthermore, the antibody is negative on platelets, granulocytes, lymphocytes, and a large number of human cell lines except the two melanoma lines MeWo and Mel 57. In cryostat sections of normal human tissue (skin, lung, liver, thymus) and of inflammatory or neoplastic tissue (cutaneous lymphoma, eczema, BCG-granuloma, and melanoma), the antibody reacts with scattered macrophages in the dermis but not with epidermal Langerhans cells, with alveolar macrophages, with liver Kupffer cells, and with scattered macrophages in the cortex and medulla of thymus. In eczema, BCG-granuloma, and cutaneous lymphoma, only a few infiltrating macrophages were stained. On the other hand, a large number of macrophages and melanophages reacted positively in melanoma. In some cases melanoma cells also stained weakly positive. Thus, the antibody detects a differentiation antigen preferentially expressed on mature, tissue-fixed macrophages and absent from blood monocytes.