Single origin of three plasmids bearing blaCTX-M-15 from different Klebsiella pneumoniae clones (Retracted article. See JUN, 2023)
Single origin of three plasmids bearing blaCTX-M-15 from different Klebsiella pneumoniae clones (Retracted article. See JUN, 2023)
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DOI：
10.1093/jac/dkt464
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发表时间：
2014-04-01
影响因子：
5.2
通讯作者：
Ko, Kwan Soo
中科院分区：
文献类型：
--
作者：
Shin, Juyoun;Ko, Kwan Soo
为了测定和比较来自三个不同肺炎克雷伯菌克隆的携带bla(CTX-M-15)的质粒的完整核苷酸序列，从三个肺炎克雷伯菌菌株中提取IncFII型质粒pKP02022、pKP09085和pKP007。这些菌株分别属于序列类型（ST）ST15、ST48 和 ST23，并在韩国分离。使用454基因组测序仪FLX系统对质粒进行测序。这三个质粒pKP02022（203577 bp）、pKP09085（213019 bp）和pKP007（246176 bp）都表现出非常相似的结构，具有类似pKPN3的主链和包括bla（OXA-1）的抗性区域， aac(6)-Ib-cr 和 cat 基因以及 bla(CTX-M-15)。它们也与先前在瑞典分离的 pUUH239.2 非常相似。在 ST23 菌株的 pKP007 中发现了铁 (III) 吸收相关基因，据报道该基因与肝脓肿有关。抗性区域包含多个插入序列，例如IS26，这可能在质粒的结构重排中发挥重要作用。从不同克隆中提取的三个质粒的结构非常相似，表明产CTX-M的肺炎克雷伯菌分离株的传播可能是由于质粒的水平转移以及随后的整合和重组所致。
To determine and compare the complete nucleotide sequences of plasmids carrying bla(CTX-M-15) from three different Klebsiella pneumoniae clones.IncFII-type plasmids pKP02022, pKP09085 and pKP007 were extracted from three K. pneumoniae strains. These strains belong to sequence types (STs) ST15, ST48 and ST23, respectively, and were isolated in Korea. Plasmids were sequenced using the 454 Genome Sequencer FLX system.The three plasmids, pKP02022 (203577 bp), pKP09085 (213019 bp) and pKP007 (246176 bp), all exhibited a very similar structure, with a pKPN3-like backbone and a resistance region including bla(OXA-1), aac(6)-Ib-cr and cat genes as well as bla(CTX-M-15). They were also very similar to pUUH239.2, previously isolated in Sweden. Iron (III) uptake-related genes were found in pKP007 from the ST23 strain, which has been reported to be associated with liver abscesses. The resistance region contained several insertion sequences, such as IS26, which may play an important role in structural rearrangements of plasmids.The very similar structure of the three plasmids, extracted from different clones, suggests that the spread of CTX-M-producing K. pneumoniae isolates might result from the horizontal transfer of plasmids and subsequent integration and recombination.