Clonal osteogenic cell lines express myogenic and adipocytic developmental potential.
Clonal osteogenic cell lines express myogenic and adipocytic developmental potential.
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克隆成骨细胞系表达肌源性和脂肪细胞发育潜力。
DOI：
10.1007/bf02556122
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发表时间：
1991
影响因子：
4.2
通讯作者：
Kahn,AJ
中科院分区：
文献类型：
--
作者：
Yamaguchi,A;Kahn,AJ
克隆成骨细胞系分离自新生大鼠颅骨，其特征在于与真实成骨细胞相关的许多功能。这些包括碱性磷酸酶活性升高（相对于成纤维细胞），PTH和PGE 2刺激的cAMP增加，1型胶原蛋白的主要合成，以及体外矿化基质蛋白的产生。通过这些标准，鉴定出具有成骨细胞样表型的5个克隆（ROB-C8 a、C11、C20、C23和C26），它们在形状、碱性磷酸酶活性水平和对PTH和PGE 2的反应性方面略有不同，C11、C20和C23对两种效应物质都有反应，而C8 a仅对PTH有反应，C26仅对PGE 2有强烈反应。在进一步检查时，还发现两个克隆（C23和C26）在达到汇合后表现出显著的肌肉肌管形成，并且三个克隆（C8 a、C11和C26）在用地塞米松处理后显示出显著的脂肪细胞分化。总之，这些数据进一步支持了（1）成骨细胞与其他结缔组织细胞的可疑个体发生关系，以及（2）与新生大鼠颅骨相关的成骨细胞处于分化和发育承诺的各个稳定阶段的概念。
Clonal osteoblastic cell lines were isolated from neonatal rat calvariae and characterized with regard to a number of features associated with authentic osteoblasts. These included elevated alkaline phosphatase activity (relative to fibroblasts), PTH and PGE2-stimulated increases in cAMP, the predominant synthesis of type 1 collagen, and the production of a mineralized matrixin vitro. By these criteria, five clones with osteoblast-like phenotypes were identified (ROB-C8a, C11, C20, C23, and C26) which varied somewhat in shape, levels of alkaline phosphatase activity, and in responsiveness to PTH and PGE2, C11, C20, and C23 responded to both effector substances, whereas C8a only responded to PTH and C26 only responded strongly to PGE2. Upon further examination, two of the clones (C23 and C26) were also found to exhibit significant muscle myotube formation after reaching confluence, and three of the clones (C8a, C11, and C26) showed marked adipocyte differentiation after treatment with dexamethasone. Overall, these data add further supporting documentation to (1) the suspected ontogenetic relationships of osteoblasts to other connective tissue cells, and (2) the concept that osteoblastic cells associated with neonatal rat calvariae are in various stable stages of differentiation and developmental commitment.