Reconstructing the evolutionary history of China: a caveat about inferences drawn from ancient DNA.
Reconstructing the evolutionary history of China: a caveat about inferences drawn from ancient DNA.
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DOI：
10.1093/molbev/msg026
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发表时间：
2003-02
影响因子：
10.7
通讯作者：
Yong-Gang Yao;Q. Kong;X. Man;H. Bandelt;Ya-ping Zhang
中科院分区：
文献类型：
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作者：
Yong-Gang Yao;Q. Kong;X. Man;H. Bandelt;Ya-ping Zhang
破译由古代线粒体DNA （mtDNA）短片段提供的贫乏信息是出了名的困难，但被认为是从遗传角度重建过去的最有希望的方法。通过单倍群特异性高可变片段（HVS）基序搜索和与现有现代数据集的匹配或接近匹配，大多数古代mtdna可以暂时分配给单倍群，这些单倍群通常是次大陆特异性的。然而，mtDNA单倍群诊断编码区多态性的进一步分型对于减少歧义建立古代样本的地理/遗传亲缘性是必不可少的。在本研究中，我们对来自中国山东泰安的76名汉族个体的mtDNA控制区的一个片段（约982 bp）进行了测序，并将这些数据与之前报道的来自山东淄博和青岛的样本相结合。对来自临淄（同一省份）的两个先前发表的古代mtDNA群体数据集的重新分析表明，古代人群与来自中国南方的现代人群具有共同的特征，而不是与欧洲mtDNA库有任何特定的亲缘关系。我们的研究结果强调，如果采用不适当的数据分析方法，在不同采样方案下获得的古代mtDNA数据和受到潜在污染的数据很容易产生在过去几千年中区域人群遗传结构发生剧烈时空变化的印象。
The decipherment of the meager information provided by short fragments of ancient mitochondrial DNA (mtDNA) is notoriously difficult but is regarded as a most promising way toward reconstructing the past from the genetic perspective. By haplogroup-specific hypervariable segment (HVS) motif search and matching or near-matching with available modern data sets, most of the ancient mtDNAs can be tentatively assigned to haplogroups, which are often subcontinent specific. Further typing for mtDNA haplogroup-diagnostic coding region polymorphisms, however, is indispensable for establishing the geographic/genetic affinities of ancient samples with less ambiguity. In the present study, we sequenced a fragment (approximately 982 bp) of the mtDNA control region in 76 Han individuals from Taian, Shandong, China, and we combined these data with previously reported samples from Zibo and Qingdao, Shandong. The reanalysis of two previously published ancient mtDNA population data sets from Linzi (same province) then indicates that the ancient populations had features in common with the modern populations from south China rather than any specific affinity to the European mtDNA pool. Our results highlight that ancient mtDNA data obtained under different sampling schemes and subject to potential contamination can easily create the impression of drastic spatiotemporal changes in the genetic structure of a regional population during the past few thousand years if inappropriate methods of data analysis are employed.