HIV type 1 envelope sequence diversity in inner city community.
HIV type 1 envelope sequence diversity in inner city community.
复制标题
市中心社区的 HIV 1 型包膜序列多样性。
DOI：
10.1089/aid.1994.10.873
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发表时间：
1994
影响因子：
1.5
通讯作者：
Hurwitz,JL
中科院分区：
文献类型：
--
作者：
Slobod,KS;Rencher,SD;Farmer,A;Smith,FS;Hurwitz,JL
Thedevastating consequences of the rapid spread of HIV have intensified efforts to develop an effective vaccine. A significant obstacle to such an effort, however, is the extremely high mutation rate of HIV. This high mutation rate, most evident in the envelope glycoprotein (gpl20), is attributed to the presence of an error-prone reverse transcriptase combined with the lack of a proofreading function. Thus, even as the infected individual makes antibody to gpl20, viral mutations occur that free the virus from neutralizing antibody activity. Highly di-verse regions encoded by the HIV-1 env gene have been identified (V1-V5), as have regions of envelope important to anti-body binding. Among the regions of Env thought to be critical to virus neutralization is a linear peptide in V3, considered by some to be the principal neutralizing determinant (PND) of Env. 1-2 Others, however, have identified dominant antibody re-sponses to distinct V and C regions of Env. 34 Thus, questions persist as to what extent antibodies recognize linear determi-nants and where selective pressure is imposed on the virus. As part of the continuing effort to assess the extent and positions of env diversity, we have initiated a study of HIV-1 isolates from residents in an inner city community. Results from this study suggest that the strongest selective pressure (within the V1-V3 region of env) in one community is targeted toward V1 and V2. We analyzed the VI to V3 env region sequences from the blood of eight HIV-1-seropositive donors contributing to a local blood bank. All donors were residents of Memphis, Tennessee. Total DNA was isolated from whole blood or
