Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm.
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomyces naeslundii Biofilm.
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DOI：
10.1016/j.joen.2015.03.005
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发表时间：
2015-08
影响因子：
4.2
通讯作者：
Bottino MC
中科院分区：
文献类型：
--
作者：
Albuquerque MT;Ryan SJ;Münchow EA;Kamocka MM;Gregory RL;Valera MC;Bottino MC
内氏放线菌已从诊断为坏死牙髓的外伤恒牙中回收。在这项工作中，提出了一种三重抗生素糊剂（TAP）模拟支架作为药物输送策略，以消除A。内氏牙本质生物膜将甲硝唑、环丙沙星和米诺环素加入到聚对二氧环己酮（PDS）聚合物溶液中，并纺成纤维支架。纤维形态，力学性能，和药物释放分别采用扫描电子显微镜，微拉伸测试，和高效液相色谱法进行了研究。将人牙本质标本（4 × 4 × 1 mm 3，n = 4/组）接种A. Naeslundii（ATCC 43146）培养7天以形成生物膜。将感染的牙本质标本暴露于TAP模拟支架、TAP溶液（阳性对照）和纯PDS（无药物支架）。牙本质感染（7天生物膜）的样本用于比较（阴性对照）。进行共聚焦激光扫描显微镜以确定细菌活力。支架显示亚微米平均纤维直径（PDS = 689 ± 312 nm和TAP模拟物= 718 ± 125 nm）。总体而言，TAP模拟支架的机械性能显著低于PDS（P ≤ 0.040）。在前24小时内，观察到所有药物的爆发释放。观察到甲硝唑和环丙沙星持续维持4周以上，但米诺环素没有。共聚焦激光扫描显微镜显示完全消除了所有暴露于TAP溶液的活菌。同时，与阴性对照和PDS相比，TAP模拟支架导致活菌百分比的显著（P <0.05）降低。我们的研究结果表明，TAP模拟支架在根除/消除细菌生物膜方面具有显着的潜力，这是再生牙髓学的关键步骤。
Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP)–mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Metronidazole, ciprofloxacin, and minocycline were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 × 4 × 1 mm3, n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens were exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Scaffolds displayed a submicron mean fiber diameter (PDS = 689 ± 312 nm and TAP-mimic = 718 ± 125 nm). Overall, TAP-mimic scaffolds showed significantly (P ≤ .040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.