Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP.
Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP.
复制标题
小鼠 DNA 结合蛋白 mC/EBP 的细胞特异性转录控制。
DOI：
10.1073/pnas.86.11.4117
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发表时间：
1989
影响因子：
11.1
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
--
作者：
Xanthopoulos,KG;Mirkovitch,J;Decker,T;Kuo,CF;DarnellJr,JE
编码小鼠C/EBP同源物的mRNA是一种参与激活肝细胞中许多基因的大鼠DNA结合蛋白，其在肝细胞中的浓度远高于大多数其他细胞，包括脾、肾、肌肉和大部分大脑。然而，脂肪细胞和肠细胞含有肝细胞25-50%的mRNA。“连续”实验表明，小鼠C/EBP mRNA的限制性细胞分布的基础是该基因的转录调节。我们还表明，破坏细胞-细胞接触事件肝细胞分散的结果在小鼠C/EBP转录迅速和广泛的减少，因为我们已经证明是一组10个基因在肝细胞特异性的方式转录的情况下。相反，打破细胞接触并将肝细胞接种在培养物中导致编码广泛分布的转录因子的Jun-B基因的转录延长增加。这些结果说明，具有有限组织分布的哺乳动物调节蛋白的表达调节在转录水平上受到控制，并且取决于细胞接触。
The mRNA encoding the mouse homolog of C/EBP, a rat DNA-binding protein that participates in activating a number of genes in hepatocytes, is present in liver cells at a far higher concentration than in most other cells, including spleen, kidney, muscle, and the majority of the brain. However, fat cells and intestinal cells contain 25-50% as much mRNA as liver cells. "Run-on" experiments show that the basis for the restricted cellular distribution of the mouse C/EBP mRNA is transcriptional regulation of the gene. We also show that disruption of cell-cell contacts incident to liver cell dispersion results in a prompt and extensive reduction in mouse C/EBP transcription as we had earlier shown to be the case for a group of 10 genes transcribed in a hepatocyte-specific fashion. In contrast, breaking cell contacts and plating the hepatocytes in culture leads to a prolonged increase in transcription of the Jun-B gene that encodes a widely distributed transcription factor. These results illustrate that the regulation of expression of a mammalian regulatory protein with limited tissue distribution is controlled at the level of transcription and depends on cell contacts.