Effect of lead on bone development and bone mass: A morphometric, densitometric, and histomorphometric study in growing rats
Effect of lead on bone development and bone mass: A morphometric, densitometric, and histomorphometric study in growing rats
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DOI：
10.1007/s002239900214
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发表时间：
1997-02-01
影响因子：
4.2
通讯作者：
Rico, H
中科院分区：
文献类型：
--
作者：
Escribano, A;Revilla, M;Rico, H
研究了铅对大鼠骨纵向发育和骨量的影响。选取35只50日龄Wistar雌性大鼠，分为对照组15只和试验组20只，每kg饲料中添加17 mg醋酸铅，连续50 d。在50 d实验结束前一天进行全身骨密度测定（TBBMC）。第50天处死大鼠，切除右股骨和第5腰椎。清洗骨骼软组织，用卡尺测量股骨长度和椎体长度，并用精密秤称所有骨骼的重量。对照组末重（P < 0.05）、TBBMC （P < 0.005）、股骨重（P < 0.005）均显著降低。各组股骨长度差异无统计学意义，但对照组第5腰椎长度大于对照组（P < 0.05）。股骨组织形态测量结果显示，添加铅组Cn-BV/TV、Tb-N、Tb-Th均低于对照组（P < 0.05）， Tb-Sp高于对照组（P < 0.05）。这些发现表明，铅诱导的轴骨发育抑制和骨量的组织形态学减少（主要由骨吸收增强引起），以及骨量的密度增加（由骨中的铅积累引起）。
The effect of exposure to lead on the longitudinal development of bone and on bone mass was studied in rats. A group of 35, 50-day-old female Wistar rats was divided into a control group of 15 rats and an experimental group of 20 rats fed a diet supplemented with 17 mg of lead acetate per kg feed for 50 days. Total body bone densitometry (TBBMC) was performed the day before ending the 50-day experiment. On day 50, all rats were killed and their right femur and 5th lumbar vertebra were dissected. The bones were cleaned of soft tissue and femoral length and vertebral length were measured with a caliper and all bones were weighed on a precision scale. Final body weight (P < 0.05), TBBMC (P < 0.005), and femur weight (P < 0.005) were significantly lower in the control group. Femur length did not differ between groups, but the length of the 5th lumbar vertebra was greater in the control group (P < 0.05). Histomorphometry of the femur showed that Cn-BV/TV, Tb-N, Tb-Th were lower (P < 0.05 in all) and Tb-Sp was higher (P < 0.05) in the group given the lead-supplemented diet. These findings suggested lead-induced inhibition of axial bone development and a histomorphometric decrease in bone mass, produced mainly by enhanced resorption, and a densitometric increase in bone mass, produced by lead accumulation in bone.