Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor
Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor
复制标题
DOI：
10.33549/physiolres.930705
复制
发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Dvorák, P
中科院分区：
文献类型：
--
作者：
Pacherník, J;Bryja, V;Dvorák, P
白血病抑制因子(LIF)是一种在多种细胞类型中表现出增殖、存活和分化的细胞因子。在这里，我们显示了LIF增强了维甲酸介导的多潜能P19胚胎癌细胞的神经诱导。LIF的这种活性表现为丰富的神经形态，随后神经特异性蛋白(N-CAM、IIIβ-微管蛋白和GA-P-43)表达增加，早期神经谱系特异性基因Mash-1上调，早期内胚层特异性基因甲胎蛋白和GATA-4下调。此外，LIF还减缓了分化细胞的生长，增加了细胞的凋亡率。
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III beta-tubulin, and GA-P-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes alpha-fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells.