Alterations in the GyrA and GyrB Subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa
Alterations in the GyrA and GyrB Subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa
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DOI：
10.1016/j.ijantimicag.2004.11.012
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发表时间：
2005-04-01
影响因子：
10.8
通讯作者：
Kim, BS
中科院分区：
文献类型：
--
作者：
Lee, JK;Lee, YS;Kim, BS
研究了103株nfxC样铜绿假单胞菌分离株中拓扑异构酶II和IV的氟喹诺酮耐药相关改变的存在。大多数nfxC样型菌株（98.1%）具有GyrA中Ile对Thr-83的取代。GyrA的单一改变（Thr-83 -> Ile）是最常见的，其次常见的改变是GyrA的Thr-83 -> Ile和ParC的Ser-87 -> Len的两个改变。在GyrA的Glin-106位发现了一个新的突变，这被认为是导致氟喹诺酮类药物耐药的原因。GyrB（Glu-468 -> Asp）和帕雷（Asp-419 -> Asn或Glu-459 -> Asp）的改变在氟喹诺酮耐药的获得中起互补作用。环丙沙星MIC与铜绿假单胞菌分离株GyrA、GyrB、ParC和帕雷中耐药相关改变的数量之间存在相关性。(C)2005年Elsevier B. V.和国际化疗学会。All rights reserved.
The presence of fluoroquinolone resistance-associated alterations in topoisomerase II and IV were investigated for 103 nfxC-like type Pseudomonas aeruginosa isolates. The most nfxC-like type isolates (98.1%) possessed the substitution of Ile for Thr-83 in GyrA. A single alteration in GyrA (Thr-83 -> Ile) was the most frequently detected and the next common alteration was two alterations with Thr-83 -> Ile in GyrA and Ser-87 -> Len in ParC. A novel alteration at position Glin-106 of GyrA, which was suggested to be responsible for fluoroquinolone resistance, was identified. Our study revealed that the alterations in GyrB (Glu-468 -> Asp) and in ParE (Asp-419 -> Asn or Glu-459 -> Asp) play a complementary role in the acquisition of resistance to fluoroquinolone. There was a correlation between the ciprofloxacin MIC and the number of resistance-associated alterations in GyrA, GyrB, ParC and ParE of P. aeruginosa isolates. (C) 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.