Nanoliter-sized liquid dispenser array for multiple biochemical analysis in microfluidic devices
Nanoliter-sized liquid dispenser array for multiple biochemical analysis in microfluidic devices
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DOI：
10.1021/ac0350007
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发表时间：
2004-02-15
影响因子：
7.4
通讯作者：
Seki, M
中科院分区：
文献类型：
--
作者：
Yamada, M;Seki, M
我们已经开发出一种由PDMS制成的微分配器阵列，其中许多纳升大小的液滴可以在气动压力下借助特定通道精确分配和混合。在这个系统中，疏水性和狭窄的通道作为一种阀门，并帮助结构液体操纵。此外，通过平行布置多个分配器，液体的单次注射变得足以制备用于不同反应的多个纳升大小的等分试样。我们设计了两种用于多种液体分配和混合的微型装置，并评估了它们的性能和再现性，证明它们足以用于定量反应。作为实际应用，使用酶促反应进行葡萄糖的生化分析。该点液技术具有消耗低、试剂和样品死体积小、操作简便等优点，可广泛应用于微量分析领域。
We have developed a microdispenser array made of PDMS, in which a number of nanoliter-sized droplets can be accurately dispensed and mixed with the aid of specific channels under pneumatic pressure. In this system, hydrophobic and narrow channels act as a kind of valve and help structural liquid manipulation. Also, by arranging multiple dispensers in parallel, a single injection of liquid becomes sufficient for the preparation of multiple nanoliter-sized aliquots for different reactions. We designed two kinds of microdevices for multiple liquid dispensing and mixing and evaluated their performance and reproducibility, proving them sufficient for quantitative reactions. As a practical application, biochemical analysis of glucose was performed using enzymatic reactions. This liquid dispensing technology can be widely applied in the field of microscale analysis due to its low consumption, small dead volume of reagents and samples, and ease of operation.