Insulin-like growth factor-I stimulates cell proliferation in the outer layer of Hertwig's epithelial root sheath and elongation of the tooth root in mouse molars in vitro
Insulin-like growth factor-I stimulates cell proliferation in the outer layer of Hertwig's epithelial root sheath and elongation of the tooth root in mouse molars in vitro
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DOI：
10.1007/s00441-004-1065-5
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Nawa, T
中科院分区：
文献类型：
--
作者：
Fujiwara, N;Tabata, MJ;Nawa, T
为了阐明根部在牙齿发育中形成的机理，我们研究了胰岛素样生长因子I（IGF-I）对5天大小鼠的下颌第一摩尔牙齿的早期根形成的作用。免疫组织化学揭示了IGF-1受体在牙齿根中的上皮根鞘（HERS）中的特定定位。随后在体外检查了IGF-I对根发育的影响，尤其是对其根源的影响。对照文化显示了她的牙周发育正常，类似于体内。然而，100 ng/ml IGF-1的存在导致其外层的细胞增殖增加，并增加了细胞的增殖。通过添加对IGF-1的抗体来消除这些影响。因此，我们建议通过调节其外层的有丝分裂活性来参与早期根部形成。
To elucidate the mechanism of root formation in tooth development, we examined the role of insulin-like growth factor I (IGF-I) on early root formation in mandibular first molar teeth from 5-day-old mice. Immunohistochemistry revealed the specific localization of the IGF-I receptor in Hertwig's epithelial root sheath (HERS) in the tooth root. The effect of IGF-I on root development, especially on HERS, was subsequently examined in vitro. The control culture showed normal development of HERS and the periodontium, resembling that in vivo. However, the presence of 100 ng/ml IGF-I resulted in elongation of HERS and increased cell proliferation in its outer layer. These effects were negated by the addition of antibodies specific for IGF-I. Thus, we propose that IGF-I is involved in early root formation by regulating the mitotic activity in the outer layer of HERS.