Characterization of the porcine acyl-CoA synthetase long-chain 4 gene and its association with growth and meat quality traits
Characterization of the porcine acyl-CoA synthetase long-chain 4 gene and its association with growth and meat quality traits
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DOI：
10.1111/j.1365-2052.2006.01436.x
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发表时间：
2006-06-01
期刊：
影响因子：
2.4
通讯作者：
Folch, J. M.
中科院分区：
文献类型：
--
作者：
Mercade, A.;Estelle, J.;Folch, J. M.
长链酰辅酶A合成酶(ACSL)催化脂肪酸、三磷酸腺苷和辅酶A生成长链酰辅酶A，激活脂肪酸进行后续反应。因此，长链酰辅酶A合成酶在脂肪生物合成和脂肪酸降解中都起着至关重要的作用。ACSL4基因被认为是位于X染色体SW2456-SW1943之间的数量性状座位(QTL)的候选基因。通过序列分析，我们鉴定了位于3‘-UTR区的10个多态性，并阐明了ACSL4的两种单倍型。在此基础上，对伊比利亚×长白杂交后代进行了ACSL4基因多态性与感兴趣性状的QTL定位和关联分析。我们报告了以前尚未发现的QTL，并描述了ACSL4基因多态与生长和油酸脂肪酸百分比的关系。最后，我们对伊比利亚猪、长白猪、大白猪、眉山猪、皮特兰猪、杜洛克猪、越南猪、佩卡里猪和巴布鲁萨猪等140头猪的等位基因频率进行了测定。
Long-chain acyl-CoA synthetase (ACSL) catalyses the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, activating fatty acids for subsequent reactions. Long-chain acyl-CoA synthetase thus plays an essential role in both lipid biosynthesis and fatty acid degradation. The ACSL4 gene was evaluated as a positional candidate gene for the quantitative trait loci (QTL) located between SW2456 and SW1943 on chromosome X. We have sequenced 4906 bp of the pig ACSL4 mRNA. Sequence analysis allowed us to identify 10 polymorphisms located in the 3'-UTR region and to elucidate two ACSL4 haplotypes. Furthermore, a QTL and an association study between polymorphisms of the ACSL4 gene and traits of interest were carried out in an Iberian x Landrace cross. We report QTL that have not been previously identified, and we describe an association of the ACSL4 polymorphisms with growth and percentage of oleic fatty acid. Finally, we have determined allelic frequencies in 140 pigs belonging to the Iberian, Landrace, Large White, Meishan, Pietrain, Duroc, Vietnamese, Peccary and Babirusa populations.