INHIBITION OF FIBRIN POLYMERIZATION BY FIBRINOGEN PROTEOLYSIS PRODUCTS
INHIBITION OF FIBRIN POLYMERIZATION BY FIBRINOGEN PROTEOLYSIS PRODUCTS
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DOI：
10.1152/ajplegacy.1962.202.4.681
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发表时间：
1962-01-01
影响因子：
--
通讯作者：
SHERRY, S
中科院分区：
文献类型：
--
作者：
LATALLO, ZS;ALKJAERSIG, N;SHERRY, S
纤维蛋白原蛋白水解产物在凝血酶抑制纤维蛋白原-纤维蛋白转化中的作用引起了人们的极大兴趣，因为它在血液凝固中可能具有重要意义。该反应已被调查后，在存在和不存在的纤维蛋白原蛋白水解产物的情况下，通过分光光度法程序的高度纯化的纤维蛋白单体的聚合。纤维蛋白原蛋白水解产物在蛋白水解产物/单体的低比率下抑制聚合，并且聚合的早期和晚期阶段同样受到影响。纤维蛋白单体的聚合速率，除了凝胶点外，在15-35[degree] C的范围内几乎不受反应温度的影响，并且由纤维蛋白原蛋白水解产物产生的抑制程度同样与温度无关。纤维蛋白原蛋白水解产物与单体的比例与聚合抑制程度呈线性关系。这表明，抑制机制涉及耦合纤维蛋白原蛋白水解产物与单体在“竞争”的方式。
The action of fibrinogen proteolysis products in inhibiting fibrinogen-fibrin conversion by thrombin has excited considerable interest because of its possible significance in blood coagulation. This reaction has been investigated by following the polymerization of highly purified fibrin monomer, in the presence and absence of fibrinogen proteolysis products, by a spectrophotometric procedure. Fibrinogen proteolysis products inhibited polymerization at low ratios of proteolysis products/monomer, and both early and late phases of polymerization were equally affected. Polymerization rates of fibrin monomer, as apart from the gel point, were little influenced by reaction temperature over the range 15-35[degree] C, and the degree of inhibition produced by fibrinogen proteolysis products was likewise independent of temperature. A linear relationship was demonstrated between the degree of inhibition to polymerization and the ratio of fibrinogen proteolysis products to monomer. It is suggested that the inhibitory mechanism involves the coupling of fibrinogen proteolysis products with monomer in a "competitive" manner.